Assessing Intervals of Epigallocatechin Gallate (EGCG) and Cannabidiol (CBD) on Saccharomyces cerevisiae Cell Reproduction as a Possible Application on Cancer Cell Proliferation

Spandana Anchoori

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Abstract

Cancer is one of the most unknown and prevalent diseases of today. There are methods to cure the disease, such as chemotherapy, however, these treatments cause a lot of pain and discomfort for patients. The purpose of this study was to find an alternative and more natural treatment to decrease the rate of cell reproduction of a cancer cell model using epigallocatechin gallate (EGCG) or cannabidiol (CBD). It was hypothesized that if Saccharomyces cerevisiae were exposed to increased concentrations of epigallocatechin gallate or cannabidiol, then the rate of cell production after they were exposed would decrease. Yeast was placed in a spectrophotometer to measure initial cell density. For CBD, the yeast was transferred to the water soluble CBD solution along with maltose needed for the fermentation process. The test tube was covered to force anaerobic respiration. For EGCG, the respective amounts of capsules were cut and the contents were poured into the test tubes and mixed in with the yeast and medium. After 24 hours, final cell densities were measured. The average difference between the initial and final absorbances for EGCG and CBD decreased as the concentrations increased. Two one-way ANOVA was run at an ��=0.05, the difference between all three concentration groups for CBD had a p-value <0.0001, rejecting the null hypothesis. The EGCG rejected the null hypothesis with a p-value of ~0.003. In conclusion, the hypothesis was supported and as levels of CBD and EGCG increase, the rate of yeast reproduction decreased.

Location

Furman Hall 209

Start Date

3-28-2020 8:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 8:30 AM

Assessing Intervals of Epigallocatechin Gallate (EGCG) and Cannabidiol (CBD) on Saccharomyces cerevisiae Cell Reproduction as a Possible Application on Cancer Cell Proliferation

Furman Hall 209

Cancer is one of the most unknown and prevalent diseases of today. There are methods to cure the disease, such as chemotherapy, however, these treatments cause a lot of pain and discomfort for patients. The purpose of this study was to find an alternative and more natural treatment to decrease the rate of cell reproduction of a cancer cell model using epigallocatechin gallate (EGCG) or cannabidiol (CBD). It was hypothesized that if Saccharomyces cerevisiae were exposed to increased concentrations of epigallocatechin gallate or cannabidiol, then the rate of cell production after they were exposed would decrease. Yeast was placed in a spectrophotometer to measure initial cell density. For CBD, the yeast was transferred to the water soluble CBD solution along with maltose needed for the fermentation process. The test tube was covered to force anaerobic respiration. For EGCG, the respective amounts of capsules were cut and the contents were poured into the test tubes and mixed in with the yeast and medium. After 24 hours, final cell densities were measured. The average difference between the initial and final absorbances for EGCG and CBD decreased as the concentrations increased. Two one-way ANOVA was run at an ��=0.05, the difference between all three concentration groups for CBD had a p-value <0.0001, rejecting the null hypothesis. The EGCG rejected the null hypothesis with a p-value of ~0.003. In conclusion, the hypothesis was supported and as levels of CBD and EGCG increase, the rate of yeast reproduction decreased.