The Isolation of a Potential Novel Antifungal Compound In Curcuma amada Rhizome Extract and Its Effect on the Growth Rate of Aspergillus niger
School Name
Spring Valley High School
Grade Level
10th Grade
Presentation Topic
Chemistry
Presentation Type
Non-Mentored
Abstract
Jeopardizing the safety and sturdiness of buildings, the fungus Aspergillus niger thrives in the heat and humidity of the Southern United States. Buildings in this region are commonly built on crawl spaces made of Southern Yellow Pine wood, which A. niger decays. With its adverse effects on human health, the abundance of A. niger is decreased through the usage of chemical-based antifungal agents which degrade the environment and harm human health. Curcuma amada is a type of mango ginger plant that has been recently investigated for its bioactive effects. This study focused on isolating a potential novel compound from C. amada extracts of three organic solvents and water, which were applied on A. niger. Paired t-tests revealed that the butanone and water-based C. amada extracts significantly inhibited the growth of A. niger compared to the control (p-value<0.0001 and p-value=0.005, respectively). The mean percentage change from butanone-based extract is 11.6% higher than the water-based treatment effect; however, the difference is not statistically significant (p-value=0.202, 95% Confidence Interval: -7.5% to 30.5%). The butanone fraction was furtherly analyzed using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), resulting in a peak at 7.18 min, containing a compound of mass 339.17. This compound is a potential novel compound. The compound's potential antifungal activity can be progressed into a natural antifungal agent for usage in buildings to reinforce building safety with a decrease in human exposure to harmful chemical agents used in fungicides.
Recommended Citation
Chen, Eileen, "The Isolation of a Potential Novel Antifungal Compound In Curcuma amada Rhizome Extract and Its Effect on the Growth Rate of Aspergillus niger" (2020). South Carolina Junior Academy of Science. 5.
https://scholarexchange.furman.edu/scjas/2020/all/5
Location
Furman Hall 108
Start Date
3-28-2020 9:30 AM
Presentation Format
Oral and Written
Group Project
No
The Isolation of a Potential Novel Antifungal Compound In Curcuma amada Rhizome Extract and Its Effect on the Growth Rate of Aspergillus niger
Furman Hall 108
Jeopardizing the safety and sturdiness of buildings, the fungus Aspergillus niger thrives in the heat and humidity of the Southern United States. Buildings in this region are commonly built on crawl spaces made of Southern Yellow Pine wood, which A. niger decays. With its adverse effects on human health, the abundance of A. niger is decreased through the usage of chemical-based antifungal agents which degrade the environment and harm human health. Curcuma amada is a type of mango ginger plant that has been recently investigated for its bioactive effects. This study focused on isolating a potential novel compound from C. amada extracts of three organic solvents and water, which were applied on A. niger. Paired t-tests revealed that the butanone and water-based C. amada extracts significantly inhibited the growth of A. niger compared to the control (p-value<0.0001 and p-value=0.005, respectively). The mean percentage change from butanone-based extract is 11.6% higher than the water-based treatment effect; however, the difference is not statistically significant (p-value=0.202, 95% Confidence Interval: -7.5% to 30.5%). The butanone fraction was furtherly analyzed using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), resulting in a peak at 7.18 min, containing a compound of mass 339.17. This compound is a potential novel compound. The compound's potential antifungal activity can be progressed into a natural antifungal agent for usage in buildings to reinforce building safety with a decrease in human exposure to harmful chemical agents used in fungicides.
