Title

The Isolation of a Potential Novel Antifungal Compound In Curcuma amada Rhizome Extract and Its Effect on the Growth Rate of Aspergillus niger

Author(s)

Eileen Chen

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Chemistry

Presentation Type

Non-Mentored

Abstract

Jeopardizing the safety and sturdiness of buildings, the fungus Aspergillus niger thrives in the heat and humidity of the Southern United States. Buildings in this region are commonly built on crawl spaces made of Southern Yellow Pine wood, which A. niger decays. With its adverse effects on human health, the abundance of A. niger is decreased through the usage of chemical-based antifungal agents which degrade the environment and harm human health. Curcuma amada is a type of mango ginger plant that has been recently investigated for its bioactive effects. This study focused on isolating a potential novel compound from C. amada extracts of three organic solvents and water, which were applied on A. niger. Paired t-tests revealed that the butanone and water-based C. amada extracts significantly inhibited the growth of A. niger compared to the control (p-value<0.0001 and p-value=0.005, respectively). The mean percentage change from butanone-based extract is 11.6% higher than the water-based treatment effect; however, the difference is not statistically significant (p-value=0.202, 95% Confidence Interval: -7.5% to 30.5%). The butanone fraction was furtherly analyzed using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), resulting in a peak at 7.18 min, containing a compound of mass 339.17. This compound is a potential novel compound. The compound's potential antifungal activity can be progressed into a natural antifungal agent for usage in buildings to reinforce building safety with a decrease in human exposure to harmful chemical agents used in fungicides.

Location

Furman Hall 108

Start Date

3-28-2020 9:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 9:30 AM

The Isolation of a Potential Novel Antifungal Compound In Curcuma amada Rhizome Extract and Its Effect on the Growth Rate of Aspergillus niger

Furman Hall 108

Jeopardizing the safety and sturdiness of buildings, the fungus Aspergillus niger thrives in the heat and humidity of the Southern United States. Buildings in this region are commonly built on crawl spaces made of Southern Yellow Pine wood, which A. niger decays. With its adverse effects on human health, the abundance of A. niger is decreased through the usage of chemical-based antifungal agents which degrade the environment and harm human health. Curcuma amada is a type of mango ginger plant that has been recently investigated for its bioactive effects. This study focused on isolating a potential novel compound from C. amada extracts of three organic solvents and water, which were applied on A. niger. Paired t-tests revealed that the butanone and water-based C. amada extracts significantly inhibited the growth of A. niger compared to the control (p-value<0.0001 and p-value=0.005, respectively). The mean percentage change from butanone-based extract is 11.6% higher than the water-based treatment effect; however, the difference is not statistically significant (p-value=0.202, 95% Confidence Interval: -7.5% to 30.5%). The butanone fraction was furtherly analyzed using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), resulting in a peak at 7.18 min, containing a compound of mass 339.17. This compound is a potential novel compound. The compound's potential antifungal activity can be progressed into a natural antifungal agent for usage in buildings to reinforce building safety with a decrease in human exposure to harmful chemical agents used in fungicides.