Title

The Effect of Potassium Chloride on Ammonia Production of Rhodospirillum rubrum

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Environmental Science

Presentation Type

Non-Mentored

Abstract

With the increasing problem of saline soil infertility, the availability of nitrogen found in this infertile soil has become a heavily researched topic. Nitrogen fixing properties are found in a wide range of bacteria. The purpose of this experiment was to evaluate whether the bacteria Rhodospirillum rubrum is an effective bacterium for aiding plants in extreme saline conditions. The hypothesis was stated that if the potassium chloride concentration was increased, then the Rhodospirillum rubrum’s nitrogenase properties would produce less ammonia because of similar inhibition properties found in other nitrogenase bacteria in the family. Three 500 ml sterile nutrient broths were prepared. 20 ml of broth was placed in 30 test tubes for the control group. In the high saline mixture, 7.2 grams of potassium chloride was added to the 600 ml of nutrient broth preceding the transfer of broth to the test tubes. The Rhodospirillum rubrum was then placed into the vials and was cultivated for 5 days. It was found that data was inconclusive and that no difference between each variable could be determined. It was however concluded that in both experimental and control groups >500 ppm of ammonia was found in a test tube filled with 20 ml of broth.

Location

HSS 214

Start Date

4-2-2022 11:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Apr 2nd, 11:30 AM

The Effect of Potassium Chloride on Ammonia Production of Rhodospirillum rubrum

HSS 214

With the increasing problem of saline soil infertility, the availability of nitrogen found in this infertile soil has become a heavily researched topic. Nitrogen fixing properties are found in a wide range of bacteria. The purpose of this experiment was to evaluate whether the bacteria Rhodospirillum rubrum is an effective bacterium for aiding plants in extreme saline conditions. The hypothesis was stated that if the potassium chloride concentration was increased, then the Rhodospirillum rubrum’s nitrogenase properties would produce less ammonia because of similar inhibition properties found in other nitrogenase bacteria in the family. Three 500 ml sterile nutrient broths were prepared. 20 ml of broth was placed in 30 test tubes for the control group. In the high saline mixture, 7.2 grams of potassium chloride was added to the 600 ml of nutrient broth preceding the transfer of broth to the test tubes. The Rhodospirillum rubrum was then placed into the vials and was cultivated for 5 days. It was found that data was inconclusive and that no difference between each variable could be determined. It was however concluded that in both experimental and control groups >500 ppm of ammonia was found in a test tube filled with 20 ml of broth.