Title

The Effect of Exposure to Lentinula edodes and Flammulina velutipes on the Biodegradation of Crystallized Polylactic Acid

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Environmental Science

Presentation Type

Non-Mentored

Abstract

Crystallized polylactic acid (CPLA) is a prevalent bioplastic which, despite being biodegradable, is extremely resistant to decomposition in natural environments. Thus, CPLA waste contamination is rapidly becoming a prevalent issue. Lentinula edodes and Flammulina velutipes are two widespread white-rot fungi whose capabilities for bioplastic degradation have not been examined. This study aimed to determine a natural approach for reducing plastic waste by evaluating the ability of L. edodes and F. velutipes to degrade CPLA bioplastic. It was hypothesized that CPLA samples would decrease in mass when individually treated with L. edodes and F. velutipes due to their similarities to previously-examined fungi such as C. versicolor and P. chrysosporium (Arikan & Bilgen, 2019; Roldán-Carrillo et al., 2003). To assess this hypothesis, 180 discs 6 mm in diameter were cut from Simple Truth™ CPLA knives and submerged in potato dextrose broth (PDB), L. edodes liquid culture, and F. velutipes liquid culture for 23 days. The initial and final masses were used to calculate the percent change in mass. An ANOVA test showed that the p-value of 0.3234 was greater than the alpha value of 0.05. Hence, the results were not significant and the hypothesis was not supported. Therefore, bioplastic waste accumulation, particularly CPLA waste, remains a prevalent issue as the material is predominantly unaltered by fungal biodegradation within 23 days.

Location

BS 349

Start Date

3-25-2023 11:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 11:15 AM

The Effect of Exposure to Lentinula edodes and Flammulina velutipes on the Biodegradation of Crystallized Polylactic Acid

BS 349

Crystallized polylactic acid (CPLA) is a prevalent bioplastic which, despite being biodegradable, is extremely resistant to decomposition in natural environments. Thus, CPLA waste contamination is rapidly becoming a prevalent issue. Lentinula edodes and Flammulina velutipes are two widespread white-rot fungi whose capabilities for bioplastic degradation have not been examined. This study aimed to determine a natural approach for reducing plastic waste by evaluating the ability of L. edodes and F. velutipes to degrade CPLA bioplastic. It was hypothesized that CPLA samples would decrease in mass when individually treated with L. edodes and F. velutipes due to their similarities to previously-examined fungi such as C. versicolor and P. chrysosporium (Arikan & Bilgen, 2019; Roldán-Carrillo et al., 2003). To assess this hypothesis, 180 discs 6 mm in diameter were cut from Simple Truth™ CPLA knives and submerged in potato dextrose broth (PDB), L. edodes liquid culture, and F. velutipes liquid culture for 23 days. The initial and final masses were used to calculate the percent change in mass. An ANOVA test showed that the p-value of 0.3234 was greater than the alpha value of 0.05. Hence, the results were not significant and the hypothesis was not supported. Therefore, bioplastic waste accumulation, particularly CPLA waste, remains a prevalent issue as the material is predominantly unaltered by fungal biodegradation within 23 days.