Title

Progression Towards A 3-Dimensional Innervation Model Of The Human Bladder

Author(s)

Elizabeth Mugo

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Mentor

Mentor: Todd Purves and Monty Hughes, Department of Pediatric Urology, Medical University of South Carolina

Oral Presentation Award

1st Place

Written Paper Award

2nd Place

Abstract

Overactive bladder is defined as difficulty with the storage of urine in the bladder causing the strong, sudden urge to urinate that can’t be controlled. Botulinum toxin (Botox) is a neurotoxin isolated from the bacterium Clostridium botulinum and is used to treat overactive bladder by injection into the bladder wall which blocks the nerves that lead to the uncontrollable urge to urinate. However, surgeons have no guide as to the location of the nerves in the bladder. The purpose of our project was to create a 3-dimensional map showing the innervation of the human bladder to serve as a surgical guide for Botox administration. Human cadaveric bladder was embedded and sectioned. Deparaffinized and dehydrated slides were incubated in primary antibody and secondary antibody conjugated with horseradish peroxidase. DAB substrate has been added which creates a stain on the slides. Slides were coversliped and scanned into single images are re-assembled into a 3-dimensional model. At the conclusion of my contribution to the project, I have stained all sections of the bladder and processed images such that they are ready to be assembled into a 3D map. It is anticipated that the bladder innervation is prominent in the trigone region of the bladder, specifically around the ureteral orifices.

Start Date

4-11-2015 9:45 AM

End Date

4-11-2015 10:00 AM

COinS
 
Apr 11th, 9:45 AM Apr 11th, 10:00 AM

Progression Towards A 3-Dimensional Innervation Model Of The Human Bladder

Overactive bladder is defined as difficulty with the storage of urine in the bladder causing the strong, sudden urge to urinate that can’t be controlled. Botulinum toxin (Botox) is a neurotoxin isolated from the bacterium Clostridium botulinum and is used to treat overactive bladder by injection into the bladder wall which blocks the nerves that lead to the uncontrollable urge to urinate. However, surgeons have no guide as to the location of the nerves in the bladder. The purpose of our project was to create a 3-dimensional map showing the innervation of the human bladder to serve as a surgical guide for Botox administration. Human cadaveric bladder was embedded and sectioned. Deparaffinized and dehydrated slides were incubated in primary antibody and secondary antibody conjugated with horseradish peroxidase. DAB substrate has been added which creates a stain on the slides. Slides were coversliped and scanned into single images are re-assembled into a 3-dimensional model. At the conclusion of my contribution to the project, I have stained all sections of the bladder and processed images such that they are ready to be assembled into a 3D map. It is anticipated that the bladder innervation is prominent in the trigone region of the bladder, specifically around the ureteral orifices.