Magnetic Hyperthermia Inactivation Of Enterotoxigenic Escherichia Coli Using Bio-Functionalized Iron-Oxide Nanoparticles

Taylor DeHart

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Microbiology

Presentation Type

Mentored

Mentor

Mentor: Jeremy Tzeng, Department of Biological Sciences, Clemson University

Oral Presentation Award

1st Place

Written Paper Award

1st Place

Abstract

Rapid emergence of new multi-drug resistant bacterial strains warrants new therapy options for treating bacterial infections caused by these strains. The purpose of this project was to use magnetic hyperthermia, in the presence of bio-functionalized iron-oxide nanoparticles (IONPs), as a non-antibiotic technique that effectively kills bacterial infections caused by enterotoxigenic Escherichia coli (ETEC), specifically E. coli ATCC 13762 (EC K99). EC K99 is known to cause bloody diarrhea in newborn pigs and calves and foodborne illnesses. The IONPs were coated with polyethylene oxide (PEO-IONPs) and bio-functionalized with specific sialic-acid glycoconjugate Neu5αc(α2-3)-Gal-(β1-4)Glcb-sp (GM3-IONPs) that mimic the receptors of EC K99 adhesins. Magnetic hyperthermia treatment of a mixture of EC K99 and GM3-IONPs resulted in a 2-log reduction of colony-forming units (CFUs) of EC K99 after 60 minutes. There also was a significant decrease in the ATP activity of EC K99 seen from luminescence assays after the hyperthermia treatment. These results suggest that magnetic hyperthermia can potentially be used as a novel non-antibiotic therapy option for treating bacterial infections.

Start Date

4-11-2015 9:45 AM

End Date

4-11-2015 10:00 AM

COinS
 
Apr 11th, 9:45 AM Apr 11th, 10:00 AM

Magnetic Hyperthermia Inactivation Of Enterotoxigenic Escherichia Coli Using Bio-Functionalized Iron-Oxide Nanoparticles

Rapid emergence of new multi-drug resistant bacterial strains warrants new therapy options for treating bacterial infections caused by these strains. The purpose of this project was to use magnetic hyperthermia, in the presence of bio-functionalized iron-oxide nanoparticles (IONPs), as a non-antibiotic technique that effectively kills bacterial infections caused by enterotoxigenic Escherichia coli (ETEC), specifically E. coli ATCC 13762 (EC K99). EC K99 is known to cause bloody diarrhea in newborn pigs and calves and foodborne illnesses. The IONPs were coated with polyethylene oxide (PEO-IONPs) and bio-functionalized with specific sialic-acid glycoconjugate Neu5αc(α2-3)-Gal-(β1-4)Glcb-sp (GM3-IONPs) that mimic the receptors of EC K99 adhesins. Magnetic hyperthermia treatment of a mixture of EC K99 and GM3-IONPs resulted in a 2-log reduction of colony-forming units (CFUs) of EC K99 after 60 minutes. There also was a significant decrease in the ATP activity of EC K99 seen from luminescence assays after the hyperthermia treatment. These results suggest that magnetic hyperthermia can potentially be used as a novel non-antibiotic therapy option for treating bacterial infections.