Enhanced selectivity for capillary zone electrophoresis using ion-pair agents

ACS Citation

Shelton, C. M.; Koch, J. T.; Desai, N.; Wheeler, J. F. Enhanced selectivity for capillary zone electrophoresis using ion-pair agents. J. Chromatogr. A 1997, 792, 455-462.

Abstract

Capillary zone electrophoresis (CZE) is unique in its simplicity and versatility for liquid separations, yet selectivity for solutes with similar charge and hydrated radius is often lacking. Conventional reversed-phase liquid chromatography (RP-LC) ion-pairing agents, ionic polymers, Group I and II metal cations and dicarboxylic acids have all been effectively used over the past decade to enhance CZE resolution through a combination of complexing effects. This review summarizes the fundamental theory of ion-“ion interaction as applied to CZE separations and discusses several of the most important and diverse applications of selectivity enhancement that utilize ion-pair equilibria. In addition, the effect of ion-pair additives on electroosmotic flow and examples where changes in analyte selectivity are unrelated to ion-“ion interaction are discussed.

Source Name

Journal of Chromatography A

Publication Date

1-1-1997

Volume

792

Issue

1/2

Page(s)

426-430

Document Type

Citation

Citation Type

Article

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