Electron‐deficient p‐benzoyl‐L‐phenylalanine Derivatives Increase Covalent Chemical Capture Yields for Protein–protein Interactions
ACS Citation
Joiner, C.M.; Breen, M.E.; Mapp, A.K. "Electron‐deficient p‐benzoyl‐L‐phenylalanine derivatives increase covalent chemical capture yields for protein–protein interactions", Protein Science, 2019, 28, 1163-1170.
Version of Record
Abstract
The photoactivatable amino acid p-benzoyl-l-phenylalanine (pBpa) has been used for the covalent capture of protein-protein interactions (PPIs) in vitro and in living cells. However, this technique often suffers from poor photocrosslinking yields due to the low reactivity of the active species. Here we demonstrate that the incorporation of halogenated pBpa analogs into proteins leads to increased crosslinking yields for protein-protein interactions. The analogs can be incorporated into live yeast and upon irradiation capture endogenous PPIs. Halogenated pBpas will extend the scope of PPIs that can be captured and expand the toolbox for mapping PPIs in their native environment.
Source Name
Protein Science
Publication Date
2019
Volume
28
Issue
6
Page(s)
1163-1170
Document Type
Article
Citation Type
Article
Comments
Joiner and Breen contributed equally to this work.