The Effects Of High Fat Diet Induced Obesity On The Translational Capacity And Efficiency In The Skeletal Muscle Of Mice
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
Obesity is an extremely common and rapidly growing medical conditions and is often a result of an unhealthy diet. Skeletal muscle is important for a healthy metabolism and regular function. Therefore, it is a fitting study model for muscular effects of obesity which disrupt the metabolic balance of protein turnover. Specifically, this study will focus on how obesity impacts translational capacity and efficiency. The purpose is to better understand what occurs metabolically within the skeletal muscles. For this experiment, three treatments of C57BL/6 mice were used. Two groups (3 and 18 months old) were fed a standard chow diet (n=10 each), the final group was 18 months old and fed a high-fat diet (n=10). The right quadriceps were tested for protein and RNA content and concentration to determine translation capacity and efficiency. Both the 18 month chow and high-fat diet (HFD) groups had significantly higher body weights from the 3 month chow treatment at sacrifice with HFD group also being significantly higher than the 18 month chow group. The 18 month chow sacrifice muscle weights were the only group that displayed significance to the younger control, (set at 0.05) despite the gap in total body weight between the HFD group and the other two groups. Relative muscle weight to body weight comparisons low muscle mass in the HFD mice. HFD mice also demonstrated severe declines in both RNA and protein concentration and content. All groups lived similar lives, so the diet is most likely to have caused these differences.
Recommended Citation
Pignone, Nathan, "The Effects Of High Fat Diet Induced Obesity On The Translational Capacity And Efficiency In The Skeletal Muscle Of Mice" (2015). South Carolina Junior Academy of Science. 108.
https://scholarexchange.furman.edu/scjas/2015/all/108
Start Date
4-11-2015 2:00 PM
End Date
4-11-2015 2:15 PM
The Effects Of High Fat Diet Induced Obesity On The Translational Capacity And Efficiency In The Skeletal Muscle Of Mice
Obesity is an extremely common and rapidly growing medical conditions and is often a result of an unhealthy diet. Skeletal muscle is important for a healthy metabolism and regular function. Therefore, it is a fitting study model for muscular effects of obesity which disrupt the metabolic balance of protein turnover. Specifically, this study will focus on how obesity impacts translational capacity and efficiency. The purpose is to better understand what occurs metabolically within the skeletal muscles. For this experiment, three treatments of C57BL/6 mice were used. Two groups (3 and 18 months old) were fed a standard chow diet (n=10 each), the final group was 18 months old and fed a high-fat diet (n=10). The right quadriceps were tested for protein and RNA content and concentration to determine translation capacity and efficiency. Both the 18 month chow and high-fat diet (HFD) groups had significantly higher body weights from the 3 month chow treatment at sacrifice with HFD group also being significantly higher than the 18 month chow group. The 18 month chow sacrifice muscle weights were the only group that displayed significance to the younger control, (set at 0.05) despite the gap in total body weight between the HFD group and the other two groups. Relative muscle weight to body weight comparisons low muscle mass in the HFD mice. HFD mice also demonstrated severe declines in both RNA and protein concentration and content. All groups lived similar lives, so the diet is most likely to have caused these differences.
Mentor
Mentor: James A. Carson, Department of Exercise Science, University of South Carolina