The Effect of Temperature on the Growth of Pseudomonas sp. and Penicillium sp. When Grown Using Motor Oil as a Source of Nutrients
School Name
Heathwood Hall Episcopal School
Grade Level
10th Grade
Presentation Topic
Microbiology
Presentation Type
Non-Mentored
Abstract
The purpose of this experiment is to determine the effect of temperature on the growth of Pseudomonas sp. and Penicillium sp. when grown using motor oil as a source of nutrients. First all of the materials needed were gathered. The bacteria and fungus were grown in an incubator at the recommended temperature while in a test tube of nutrient broth. After they grew for two weeks, thirty six test tubes were filled with two milliliters of oil and seven milliliters of water. A pipet was used to transfer one milliliter of nutrient broth/bacteria mixture to eighteen of the test tubes. One milliliter of nutrient broth/fungus mixture was added to the rest of the test tubes using a different pipet. Three sets of twelve containing six bacteria and six fungus test tubes were separated and put into three different climates: cold, room temperature, and hot. They were left to grow using the oil as a source of nutrients for two weeks. After these two weeks bacteria and fungus had grown directly underneath the layer of oil at the top of the water. The amount of growth was measured in millimeters for each test tube. The results of the experiment showed that in each of the climates the average growth of the two microorganisms were different than the microorganisms in the other climates. In conclusion, the results supported the null hypothesis, stating there will be no difference in growth in any of the climates, to be rejected.
Recommended Citation
Byrd, Grayson B., "The Effect of Temperature on the Growth of Pseudomonas sp. and Penicillium sp. When Grown Using Motor Oil as a Source of Nutrients" (2015). South Carolina Junior Academy of Science. 255.
https://scholarexchange.furman.edu/scjas/2015/all/255
Start Date
4-11-2015 11:30 AM
End Date
4-11-2015 11:45 AM
The Effect of Temperature on the Growth of Pseudomonas sp. and Penicillium sp. When Grown Using Motor Oil as a Source of Nutrients
The purpose of this experiment is to determine the effect of temperature on the growth of Pseudomonas sp. and Penicillium sp. when grown using motor oil as a source of nutrients. First all of the materials needed were gathered. The bacteria and fungus were grown in an incubator at the recommended temperature while in a test tube of nutrient broth. After they grew for two weeks, thirty six test tubes were filled with two milliliters of oil and seven milliliters of water. A pipet was used to transfer one milliliter of nutrient broth/bacteria mixture to eighteen of the test tubes. One milliliter of nutrient broth/fungus mixture was added to the rest of the test tubes using a different pipet. Three sets of twelve containing six bacteria and six fungus test tubes were separated and put into three different climates: cold, room temperature, and hot. They were left to grow using the oil as a source of nutrients for two weeks. After these two weeks bacteria and fungus had grown directly underneath the layer of oil at the top of the water. The amount of growth was measured in millimeters for each test tube. The results of the experiment showed that in each of the climates the average growth of the two microorganisms were different than the microorganisms in the other climates. In conclusion, the results supported the null hypothesis, stating there will be no difference in growth in any of the climates, to be rejected.
