Effect Of Interleukin-6 On Translational Capacity In C57Bl/6 And Apcmin/+ Mice

Mayank Patel

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Mentor

Mentor: James A. Carson, Department of Exercise Science, University of South Carolina

Abstract

Cancer cachexia is a multifactorial condition that results in skeletal muscle atrophy; it is responsible for 25 to 30% of all cancer-related deaths. Interleukin 6 (IL-6) is a pro-inflammatory cytokine that has been widely studied as a factor in cachexia development and has potential as a therapeutic target. It has been shown that when IL-6 is overexpressed in male Apcmin/+ (min) mice, protein synthesis will decrease. The purpose of this experiment was to examine the effect of IL-6 on muscle protein and RNA content in female wild type (C57BL/6) and min mice. It was hypothesized that min mice would show decreased protein synthesis in response to IL-6 overexpression. Four groups of mice were examined: B6, B6 + IL6, Min, and Min + IL6. To determine protein and RNA concentrations per unit of muscle, the Fleck Munro Assay was performed. At the time of sacrifice, Apcmin/+ mice had significantly more body weight loss than B6 mice. Min mice lost an average of 4% body weight, while B6 had no change in body weight. There was no significant (two-way ANOVA, p = 0.05) difference between min and min + IL-6 in quadriceps RNA concentration. Preliminary analysis revealed a significant (two-way ANOVA, p = 0.05) difference between B6 and B6 + IL-6 mice, with B6+IL-6 showing and increase in RNA concentration. No difference was seen between min and min + IL-6 mice. IL-6 overexpression induces an increase in RNA concentration in B6 mice, while a suppression in min + IL-6 mice.

Start Date

4-11-2015 10:00 AM

End Date

4-11-2015 10:15 AM

COinS
 
Apr 11th, 10:00 AM Apr 11th, 10:15 AM

Effect Of Interleukin-6 On Translational Capacity In C57Bl/6 And Apcmin/+ Mice

Cancer cachexia is a multifactorial condition that results in skeletal muscle atrophy; it is responsible for 25 to 30% of all cancer-related deaths. Interleukin 6 (IL-6) is a pro-inflammatory cytokine that has been widely studied as a factor in cachexia development and has potential as a therapeutic target. It has been shown that when IL-6 is overexpressed in male Apcmin/+ (min) mice, protein synthesis will decrease. The purpose of this experiment was to examine the effect of IL-6 on muscle protein and RNA content in female wild type (C57BL/6) and min mice. It was hypothesized that min mice would show decreased protein synthesis in response to IL-6 overexpression. Four groups of mice were examined: B6, B6 + IL6, Min, and Min + IL6. To determine protein and RNA concentrations per unit of muscle, the Fleck Munro Assay was performed. At the time of sacrifice, Apcmin/+ mice had significantly more body weight loss than B6 mice. Min mice lost an average of 4% body weight, while B6 had no change in body weight. There was no significant (two-way ANOVA, p = 0.05) difference between min and min + IL-6 in quadriceps RNA concentration. Preliminary analysis revealed a significant (two-way ANOVA, p = 0.05) difference between B6 and B6 + IL-6 mice, with B6+IL-6 showing and increase in RNA concentration. No difference was seen between min and min + IL-6 mice. IL-6 overexpression induces an increase in RNA concentration in B6 mice, while a suppression in min + IL-6 mice.