Mexicanin I Inhibits Fibrocyte Generation From Mouse Splenocytes Accompanied By Down Regulation Of KLF4

Maya Rush

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Mentor

Mentor: Walden Ai, Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine

Abstract

Tumor metastasis is a multiple step process which affects all cancer patients. Previous research in Dr. Walden Ai’s lab at the University of South Carolina’s School of Medicine showed that deficiency of kruppel-like factor 4 (KLF4), a transcription factor, reduced tumor lung metastasis and was associated with decreased fibrocyte generation from splenocytes. Given that there is a strong association between fibrocyte levels and tumor metastasis, it was hypothesized that the inhibition of KLF4 would reduce fibrocyte generation leading to decreased tumor metastasis. Mexicanin I and C188-9 were used to assess the inhibitory effect on KLF4 and fibrocyte generation from mouse splenocytes. The expression levels of KLF4 downstream targets were examined by real-time PCR and Western blotting. Both Mexicanin I and C188-9 inhibited KLF4 expression and fibrocyte generation, but Mexicanin I was more efficient. Microscopic examination revealed that the fibrocyte generation was far less expressed when treated with 0.1 μL of Mexicanin I, compared to 2 μL of C188-9. When the splenocytes were treated with 0.1 μM of Mexicanin I there was one fibrocyte cell. After being treated with 2 μM of the C188-9 there was only 1 spindle like cell left. Real time PCR revealed that the expression of fibroblast specific protein-1 (FSP-1) and CXCR4 was notably reduced with the treatment of Mexicanin I. The KLF4 inhibitor Mexicanin I inhibits fibrocyte generation from murine splenocytes.

Start Date

4-11-2015 9:30 AM

End Date

4-11-2015 9:45 AM

COinS
 
Apr 11th, 9:30 AM Apr 11th, 9:45 AM

Mexicanin I Inhibits Fibrocyte Generation From Mouse Splenocytes Accompanied By Down Regulation Of KLF4

Tumor metastasis is a multiple step process which affects all cancer patients. Previous research in Dr. Walden Ai’s lab at the University of South Carolina’s School of Medicine showed that deficiency of kruppel-like factor 4 (KLF4), a transcription factor, reduced tumor lung metastasis and was associated with decreased fibrocyte generation from splenocytes. Given that there is a strong association between fibrocyte levels and tumor metastasis, it was hypothesized that the inhibition of KLF4 would reduce fibrocyte generation leading to decreased tumor metastasis. Mexicanin I and C188-9 were used to assess the inhibitory effect on KLF4 and fibrocyte generation from mouse splenocytes. The expression levels of KLF4 downstream targets were examined by real-time PCR and Western blotting. Both Mexicanin I and C188-9 inhibited KLF4 expression and fibrocyte generation, but Mexicanin I was more efficient. Microscopic examination revealed that the fibrocyte generation was far less expressed when treated with 0.1 μL of Mexicanin I, compared to 2 μL of C188-9. When the splenocytes were treated with 0.1 μM of Mexicanin I there was one fibrocyte cell. After being treated with 2 μM of the C188-9 there was only 1 spindle like cell left. Real time PCR revealed that the expression of fibroblast specific protein-1 (FSP-1) and CXCR4 was notably reduced with the treatment of Mexicanin I. The KLF4 inhibitor Mexicanin I inhibits fibrocyte generation from murine splenocytes.