Optical Probes For Noninvasive Molecular Imaging Of Cancer Biomarkers In Glioblastoma Cell Lines
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
We designed and created non-invasive, near infra-red (NIR) optical imaging probes targeted to specific biomarkers in Glioblastoma (GBM) cell lines. Through western blot analysis, specific proteins were observed to be consistently overexpressed in the GBM cell lines. These unique biomarkers include: transferrin receptor (Tfr) proteins, epidermal growth factor receptor (EGFr) proteins, and platelet derived growth factor receptor (PDGFr) proteins. Probes were created out of short peptides with ends complimentary to the amino acid sequence on the binding site of each respective protein receptor. A fluorescent dye (Dylight- 680) was attached to the free amine group of each peptide. We observed and quantified the uptake of these conjugates through fluorescent microscopy in an effort to optimize the efficiency of the treatments in preparation for use in vivo.
Recommended Citation
Mitchell, Faith, "Optical Probes For Noninvasive Molecular Imaging Of Cancer Biomarkers In Glioblastoma Cell Lines" (2015). South Carolina Junior Academy of Science. 81.
https://scholarexchange.furman.edu/scjas/2015/all/81
Start Date
4-11-2015 11:15 AM
End Date
4-11-2015 11:30 AM
Optical Probes For Noninvasive Molecular Imaging Of Cancer Biomarkers In Glioblastoma Cell Lines
We designed and created non-invasive, near infra-red (NIR) optical imaging probes targeted to specific biomarkers in Glioblastoma (GBM) cell lines. Through western blot analysis, specific proteins were observed to be consistently overexpressed in the GBM cell lines. These unique biomarkers include: transferrin receptor (Tfr) proteins, epidermal growth factor receptor (EGFr) proteins, and platelet derived growth factor receptor (PDGFr) proteins. Probes were created out of short peptides with ends complimentary to the amino acid sequence on the binding site of each respective protein receptor. A fluorescent dye (Dylight- 680) was attached to the free amine group of each peptide. We observed and quantified the uptake of these conjugates through fluorescent microscopy in an effort to optimize the efficiency of the treatments in preparation for use in vivo.
Mentor
Mentor: Steven Ornstein, Department of Biology, Medical University of South Carolina