High Speed 3D Imaging With Two-Photon Light Sheet Microscopy
School Name
Academic Magnet High School
Grade Level
12th Grade
Presentation Topic
Engineering
Presentation Type
Mentored
Abstract
The current image speed of multi-photon microscopy is not enough for fast biological processes such as molecular diffusion, so improvements were made to existing optical designs. Two-photon microscopy and light sheet fluorescent microscopy were combined and used to create the two-photon light sheet microscope which offers optical sectioning at a much faster speed than previous designs. The capabilities of the microscope were used to perform the first three-dimensional Fluorescent Recovery After Photobleaching experiment, as well as image cardiomyocyte spheroid cells. The microscope is shown to be the fastest method for imaging fast biological processes in three-dimensions.
Recommended Citation
Chaplin, Christopher, "High Speed 3D Imaging With Two-Photon Light Sheet Microscopy" (2016). South Carolina Junior Academy of Science. 1.
https://scholarexchange.furman.edu/scjas/2016/all/1
Location
Owens G07
Start Date
4-16-2016 12:00 PM
High Speed 3D Imaging With Two-Photon Light Sheet Microscopy
Owens G07
The current image speed of multi-photon microscopy is not enough for fast biological processes such as molecular diffusion, so improvements were made to existing optical designs. Two-photon microscopy and light sheet fluorescent microscopy were combined and used to create the two-photon light sheet microscope which offers optical sectioning at a much faster speed than previous designs. The capabilities of the microscope were used to perform the first three-dimensional Fluorescent Recovery After Photobleaching experiment, as well as image cardiomyocyte spheroid cells. The microscope is shown to be the fastest method for imaging fast biological processes in three-dimensions.
Mentor
Mentor: Dr. Tong Ye; Clemson/MUSC