Amplifying Calcium/Calmodulin Dependent Protein Kinase Ii Delta Variant 9 In Healthy And Dilated Cardiomyopathy Human Cardiac Fibroblasts

Savanah Dale

School Name

Governor's School for Science and Math

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: Dr. McDermott; Department of Medicine, Medical University of South Carolina

Abstract

Human Cardiac Fibroblasts make up the majority of cells in the human heart. These cells contain Calcium/Calmodulin Dependent Protein Kinase II Delta (CAMKIID). This protein kinase it codes for is activated by a calcium modulated protein, calmodulin, and it targets calcium ion channels in heart muscle tissues, which allows the release of calcium ions that are necessary for muscle contraction. CAMKIID variants 2 and 3 have been linked to dilated cardiomyopathy in previous studies with mouse cardiac fibroblasts. Dilated cardiomyopathy is a disease in which the left ventricle of the heart enlarges, stretching the muscle and causing the heart to become less efficient; this is the leading cause of heart failure. CAMKIID variant 9 is expressed in mouse cardiac fibroblasts, but it is unknown if it is expressed in human cardiac fibroblasts, nor is it known if it correlates to dilated cardiomyopathy. We used Reverse-Transcriptase Polymerase to determine if CAMKIID variant 9 is expressed in human cardiac fibroblasts. We hypothesized that CAMKIID variant 9 would be expressed in the human cardiac fibroblasts. CAMKIID variant 9 was expressed in both healthy human cardiac fibroblasts as well as in human cardiac fibroblasts affected by dilated cardiomyopathy. Knowing that CAMKIID variant 9 is expressed in cardiac fibroblasts will facilitate future research to determine its role in the development of dilated cardiomyopathy.

Location

Owens 201

Start Date

4-16-2016 9:45 AM

COinS
 
Apr 16th, 9:45 AM

Amplifying Calcium/Calmodulin Dependent Protein Kinase Ii Delta Variant 9 In Healthy And Dilated Cardiomyopathy Human Cardiac Fibroblasts

Owens 201

Human Cardiac Fibroblasts make up the majority of cells in the human heart. These cells contain Calcium/Calmodulin Dependent Protein Kinase II Delta (CAMKIID). This protein kinase it codes for is activated by a calcium modulated protein, calmodulin, and it targets calcium ion channels in heart muscle tissues, which allows the release of calcium ions that are necessary for muscle contraction. CAMKIID variants 2 and 3 have been linked to dilated cardiomyopathy in previous studies with mouse cardiac fibroblasts. Dilated cardiomyopathy is a disease in which the left ventricle of the heart enlarges, stretching the muscle and causing the heart to become less efficient; this is the leading cause of heart failure. CAMKIID variant 9 is expressed in mouse cardiac fibroblasts, but it is unknown if it is expressed in human cardiac fibroblasts, nor is it known if it correlates to dilated cardiomyopathy. We used Reverse-Transcriptase Polymerase to determine if CAMKIID variant 9 is expressed in human cardiac fibroblasts. We hypothesized that CAMKIID variant 9 would be expressed in the human cardiac fibroblasts. CAMKIID variant 9 was expressed in both healthy human cardiac fibroblasts as well as in human cardiac fibroblasts affected by dilated cardiomyopathy. Knowing that CAMKIID variant 9 is expressed in cardiac fibroblasts will facilitate future research to determine its role in the development of dilated cardiomyopathy.