The difference in amount of ethanol produced by Portobello and Shiitake mushroom cellulose
School Name
Heathwood Hall
Grade Level
9th Grade
Presentation Topic
Biochemistry
Presentation Type
Non-Mentored
Abstract
Two common types of mushrooms, the Portobello and Shiitake mushrooms, were used to see which mushroom produced more cellulosic ethanol. The hypothesis was if the enzyme Cellobiase reacted with cellulose from Shiitake and Portobello mushrooms, then Portobello would produce more ethanol. The results of this study did support the hypothesis. In this study Portobello mushrooms produced almost 20 times more ethanol. A Bio Rad Labs Biofuel Enzyme Kit was used to measure the amount of p-Nitrophenol produced by these mushrooms. p-Nitrophenol was then used as an indicator of a proportional production of ethanol. This result means that there is a potential to use Portobello mushrooms to process cellulosic ethanol for possibly making fuel for vehicles in the future.
Recommended Citation
Haywood, Riley and Schumacher, Noah, "The difference in amount of ethanol produced by Portobello and Shiitake mushroom cellulose" (2017). South Carolina Junior Academy of Science. 10.
https://scholarexchange.furman.edu/scjas/2017/all/10
Location
Wall 118
Start Date
3-25-2017 11:30 AM
Presentation Format
Oral and Written
Group Project
Yes
The difference in amount of ethanol produced by Portobello and Shiitake mushroom cellulose
Wall 118
Two common types of mushrooms, the Portobello and Shiitake mushrooms, were used to see which mushroom produced more cellulosic ethanol. The hypothesis was if the enzyme Cellobiase reacted with cellulose from Shiitake and Portobello mushrooms, then Portobello would produce more ethanol. The results of this study did support the hypothesis. In this study Portobello mushrooms produced almost 20 times more ethanol. A Bio Rad Labs Biofuel Enzyme Kit was used to measure the amount of p-Nitrophenol produced by these mushrooms. p-Nitrophenol was then used as an indicator of a proportional production of ethanol. This result means that there is a potential to use Portobello mushrooms to process cellulosic ethanol for possibly making fuel for vehicles in the future.
