a comparison of the total polyphenols in Daucus carota, Cyanococcus, Citrus sinensis, Citrus limon, actinidia deliciosa, and Mangifera indica
School Name
Spring Valley High School
Grade Level
10th Grade
Presentation Topic
Biochemistry
Presentation Type
Non-Mentored
Written Paper Award
2nd Place
Abstract
Degenerative diseases, as well as bacterial infections are caused by oxidative stress, and are a huge problem in the world. Antioxidants are needed to safely balance the harmful effects of free radicals without causing any damage to the body. Bioactive compounds, such as polyphenols and polyphenol derivatives, have powerful antioxidative abilities and can treat and prevent degenerative diseases. Many common edible plants, such as carrot, blueberry, lemon, kiwi, orange, and mango contain polyphenols. Polyphenols can measured by creating an aqueous extract. This can be done by boiling down each fruit or vegetable in water. 5 mL of the resulting extract were mixed with 5 mL of Folin-Denis reagent and heated on a hot plate for a minute. The resulting solution was transferred into a cuvette and placed into a spectrovis that was calibrated to a blank of Folin-Denis reagent. The extracts were measured for absorbance at a wavelength of 450 nm to determine relative polyphenol levels. An ANOVA test at alpha = 0.05 (F(5)=118.651, p = > 0.001) was used to test the significance of the results. Since the ANOVA test returned a p-value of less than 0.001, the data is significant because 0.001 is less than the alpha value. This means the null hypothesis that there is no difference between the extracts is rejected, as there was significant differences in the polyphenol levels. The hypothesis that the darkest colored extract, in this case blueberry, would have the highest level of polyphenols was also supported by the data.
Recommended Citation
Kumar, Vikram, "a comparison of the total polyphenols in Daucus carota, Cyanococcus, Citrus sinensis, Citrus limon, actinidia deliciosa, and Mangifera indica" (2017). South Carolina Junior Academy of Science. 12.
https://scholarexchange.furman.edu/scjas/2017/all/12
Location
Wall 118
Start Date
3-25-2017 11:15 AM
Presentation Format
Oral and Written
Group Project
No
a comparison of the total polyphenols in Daucus carota, Cyanococcus, Citrus sinensis, Citrus limon, actinidia deliciosa, and Mangifera indica
Wall 118
Degenerative diseases, as well as bacterial infections are caused by oxidative stress, and are a huge problem in the world. Antioxidants are needed to safely balance the harmful effects of free radicals without causing any damage to the body. Bioactive compounds, such as polyphenols and polyphenol derivatives, have powerful antioxidative abilities and can treat and prevent degenerative diseases. Many common edible plants, such as carrot, blueberry, lemon, kiwi, orange, and mango contain polyphenols. Polyphenols can measured by creating an aqueous extract. This can be done by boiling down each fruit or vegetable in water. 5 mL of the resulting extract were mixed with 5 mL of Folin-Denis reagent and heated on a hot plate for a minute. The resulting solution was transferred into a cuvette and placed into a spectrovis that was calibrated to a blank of Folin-Denis reagent. The extracts were measured for absorbance at a wavelength of 450 nm to determine relative polyphenol levels. An ANOVA test at alpha = 0.05 (F(5)=118.651, p = > 0.001) was used to test the significance of the results. Since the ANOVA test returned a p-value of less than 0.001, the data is significant because 0.001 is less than the alpha value. This means the null hypothesis that there is no difference between the extracts is rejected, as there was significant differences in the polyphenol levels. The hypothesis that the darkest colored extract, in this case blueberry, would have the highest level of polyphenols was also supported by the data.
