Optimization of Bivector Systems for Export of Isoprenoids In Biofuel Applications
School Name
Governor's School for Science & Mathematics
Grade Level
12th Grade
Presentation Topic
Microbiology
Presentation Type
Mentored
Abstract
As the need for alternative energy sources has increased, many scientists have delved further into the production of biofuels, which are energy sources derived directly from living matter. In this research project, an economically efficient way to mass produce and harvest biofuels was explored. Escherichia coli DH10B transformed with plasmids encoding isoprenoids was used in the experiment for their production. The isoprenoid used in this part of the project was canthaxanthin. Using an Acridine Orange Assay, an attempt to validate the efflux pump activity encoded by plasmid EcoMsbA was made. E. coli DH10B with EcoMsbA and the control both did not show any fluorescence, indicating an issue. After testing the Assay, it was concluded that it was no longer good and does not cause fluorescence to be observed. Through the canthaxanthin protocol, the amount of canthaxanthin present could be calculated based on the absorbance at 460 nm.
Recommended Citation
Malik, Samah, "Optimization of Bivector Systems for Export of Isoprenoids In Biofuel Applications" (2017). South Carolina Junior Academy of Science. 153.
https://scholarexchange.furman.edu/scjas/2017/all/153
Location
Wall 224
Start Date
3-25-2017 11:30 AM
Presentation Format
Oral and Written
Group Project
No
Optimization of Bivector Systems for Export of Isoprenoids In Biofuel Applications
Wall 224
As the need for alternative energy sources has increased, many scientists have delved further into the production of biofuels, which are energy sources derived directly from living matter. In this research project, an economically efficient way to mass produce and harvest biofuels was explored. Escherichia coli DH10B transformed with plasmids encoding isoprenoids was used in the experiment for their production. The isoprenoid used in this part of the project was canthaxanthin. Using an Acridine Orange Assay, an attempt to validate the efflux pump activity encoded by plasmid EcoMsbA was made. E. coli DH10B with EcoMsbA and the control both did not show any fluorescence, indicating an issue. After testing the Assay, it was concluded that it was no longer good and does not cause fluorescence to be observed. Through the canthaxanthin protocol, the amount of canthaxanthin present could be calculated based on the absorbance at 460 nm.
Mentor
Mentor: Tzuen-Rong Tzeng, Clemson University