The Effect of Antibiotics on the Protein Changes in E. coli.
School Name
Heathwood Hall
Grade Level
10th Grade
Presentation Topic
Microbiology
Presentation Type
Non-Mentored
Oral Presentation Award
1st Place
Abstract
The purpose of this research is to study the relationship between E. coli exposure to Penicillin and the protein changes taking place when complete antibiotic resistance is achieved, as well to test the concept of whether there is a relationship between protein changes and antibiotic resistance. The type of bacteria that was used was E. coli, and there were 4 different cultures that each built up resistance independent of the others. Antibiotic resistant bacteria are an emerging problem in developed and developing countries, so this research aims to provide insight into a possible link between antibiotic resistance and bacterial proteins. The E. coli strain used was OP50 and the E. coli was spread onto petri dish plates filled with RAPID'E.coli 2 Agar, and then a Penicillin disk was immediately placed onto each plate. After the 4th generation of repeating this process, the bacteria were collected and gathered for an SDS-PAGE protein electrophoresis. The hypothesis for this experiment was that if the E. coli bacteria are repeatedly exposed to the Penicillin antibiotic, then the surviving bacteria will exhibit resistance to Penicillin and a change in their protein structure. The experiment presented limited results that were not sufficient evidence to support my hypothesis or null hypothesis. In conclusion, the results suggest the methodology used is valid, but there was not a sufficient amount of data collected to prove or disprove the idea that protein changes in a bacteria can be linked to the exposure to Penicillin that these bacteria endured.
Recommended Citation
Mathews, Ben, "The Effect of Antibiotics on the Protein Changes in E. coli." (2017). South Carolina Junior Academy of Science. 161.
https://scholarexchange.furman.edu/scjas/2017/all/161
Location
Wall 224
Start Date
3-25-2017 10:15 AM
Presentation Format
Oral and Written
Group Project
No
The Effect of Antibiotics on the Protein Changes in E. coli.
Wall 224
The purpose of this research is to study the relationship between E. coli exposure to Penicillin and the protein changes taking place when complete antibiotic resistance is achieved, as well to test the concept of whether there is a relationship between protein changes and antibiotic resistance. The type of bacteria that was used was E. coli, and there were 4 different cultures that each built up resistance independent of the others. Antibiotic resistant bacteria are an emerging problem in developed and developing countries, so this research aims to provide insight into a possible link between antibiotic resistance and bacterial proteins. The E. coli strain used was OP50 and the E. coli was spread onto petri dish plates filled with RAPID'E.coli 2 Agar, and then a Penicillin disk was immediately placed onto each plate. After the 4th generation of repeating this process, the bacteria were collected and gathered for an SDS-PAGE protein electrophoresis. The hypothesis for this experiment was that if the E. coli bacteria are repeatedly exposed to the Penicillin antibiotic, then the surviving bacteria will exhibit resistance to Penicillin and a change in their protein structure. The experiment presented limited results that were not sufficient evidence to support my hypothesis or null hypothesis. In conclusion, the results suggest the methodology used is valid, but there was not a sufficient amount of data collected to prove or disprove the idea that protein changes in a bacteria can be linked to the exposure to Penicillin that these bacteria endured.
