The Effects of SSRIs on Premature Osteoblasts

School Name

Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Mentor

Mentor: Amanda LaRue, Medical University of South Carolina

Abstract

Half of all war veterans experience musculoskeletal extremity injuries. These injuries can become delayed or nonunion. Current treatments for these types of fractures are only moderately successful. Bone grafts and metal nails do not always heal completely or stay uninfected. Therefore, there is a need for new therapeutic methods for orthopedic injuries. Hematopoietic stem cells and mesenchymal stems cells have shown complete bone formation. Over sixty percent of these war veterans are on a prescribed antidepressant, most commonly a selective serotonin reuptake inhibitor, for depression or depressive-associated disorders like Post Traumatic Stress Syndrome, Insomnia, or Attention Deficit Hyperactivity Disorder. Antidepressants, particularly selective serotonin reuptake inhibitors (SSRIs), may pose a threat to complete orthopedic healing. The mechanistic effects of SSRIs on bone fracture healing are largely unknown. However, preliminary data suggest that SSRIs inhibit bone healing in a calvarial critical size defect model by 4 weeks in vivo. It is clinically significant, not only to war veterans, but to anyone who is prescribed an antidepressant and is at a high risk for an orthopedic injury. It was hypothesized that after treating the premature osteoblast calvaria cell line MC3T3-E1 subclone 4 cells with Paroxetine and Sertraline (the two most commonly prescribed SSRIs), it will be shown that SSRIs inhibit premature osteoblast differentiation in vitro.

Start Date

3-25-2017 11:59 PM

Presentation Format

Written Only

Group Project

No

COinS
 
Mar 25th, 11:59 PM

The Effects of SSRIs on Premature Osteoblasts

Half of all war veterans experience musculoskeletal extremity injuries. These injuries can become delayed or nonunion. Current treatments for these types of fractures are only moderately successful. Bone grafts and metal nails do not always heal completely or stay uninfected. Therefore, there is a need for new therapeutic methods for orthopedic injuries. Hematopoietic stem cells and mesenchymal stems cells have shown complete bone formation. Over sixty percent of these war veterans are on a prescribed antidepressant, most commonly a selective serotonin reuptake inhibitor, for depression or depressive-associated disorders like Post Traumatic Stress Syndrome, Insomnia, or Attention Deficit Hyperactivity Disorder. Antidepressants, particularly selective serotonin reuptake inhibitors (SSRIs), may pose a threat to complete orthopedic healing. The mechanistic effects of SSRIs on bone fracture healing are largely unknown. However, preliminary data suggest that SSRIs inhibit bone healing in a calvarial critical size defect model by 4 weeks in vivo. It is clinically significant, not only to war veterans, but to anyone who is prescribed an antidepressant and is at a high risk for an orthopedic injury. It was hypothesized that after treating the premature osteoblast calvaria cell line MC3T3-E1 subclone 4 cells with Paroxetine and Sertraline (the two most commonly prescribed SSRIs), it will be shown that SSRIs inhibit premature osteoblast differentiation in vitro.