The Effects of Polycyclic aromatic Hydrocarbons on the Production of Lactate Dehydrogenase in MCF-7 Cells

School Name

Dutch Fork High School

Grade Level

11th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Mentor

Mentor: Dr. Samir Raychoudhury, Benedict College

Abstract

PAHs are compounds that are toxic to human beings that is found in soot, tar mineral oils and many other places in the environment. An abundance of research has been conducted on PAHs as potent carcinogens for many types of cancers. The subject of this study is how PAHs react in comparison to other chemicals (Tamoxifen and Estradiol) in MCF-7 breast cancer cells. The experiments conducted involve the effect of high and low doses of PAHs in relation to the MCF-7 cell line of breast cancer cells. These doses will be compared in relation to both controls as well as Tamoxifen, a competitive inhibitor to estrogen receptors in MCF-7. If a cell is damaged, it will release lactate dehydrogenase, so this will be the dependent variable to measure cell damage. The hypothesis is that when the high and low doses of PAH are exposed to MCF-7 cells, they will release higher amounts of LDH as opposed to Tamoxifen and the controls tested.

Location

Wall 118

Start Date

3-25-2017 9:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 9:15 AM

The Effects of Polycyclic aromatic Hydrocarbons on the Production of Lactate Dehydrogenase in MCF-7 Cells

Wall 118

PAHs are compounds that are toxic to human beings that is found in soot, tar mineral oils and many other places in the environment. An abundance of research has been conducted on PAHs as potent carcinogens for many types of cancers. The subject of this study is how PAHs react in comparison to other chemicals (Tamoxifen and Estradiol) in MCF-7 breast cancer cells. The experiments conducted involve the effect of high and low doses of PAHs in relation to the MCF-7 cell line of breast cancer cells. These doses will be compared in relation to both controls as well as Tamoxifen, a competitive inhibitor to estrogen receptors in MCF-7. If a cell is damaged, it will release lactate dehydrogenase, so this will be the dependent variable to measure cell damage. The hypothesis is that when the high and low doses of PAH are exposed to MCF-7 cells, they will release higher amounts of LDH as opposed to Tamoxifen and the controls tested.