E4 Domains and Their Anti-Fibrotic Nature

School Name

Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: Carol Feghali-Bostwick, Medical University of South Carolina

Oral Presentation Award

4th Place

Abstract

Scleroderma is an autoimmune disease that has a high death rate due to fibrosis. Recently a peptide called E4 has been identified to lessen the effects and even treat fibrosis. However, this peptide is difficult and costly to synthesize. Therefore, research has been conducted to identify a smaller active domain of E4 to be used in treatments. This experiment was conducted over a three week period and began with the culturing of three cells lines: NL-98, NL-88, and NL-82. After, all of the cells lines were treated with TGF and/or peptide domains and harvested into cell lysates and supernatants. The lysates were then probed for GAPDH, Collagen 1A1, MMP, and Fibronectin, while the supernatants were probed for just Collagen 1A1, MMP, and Fibronectin. The data from the Westen Blots were analyzed using densitometry. The final conclusion after looking at the resulting graphs is that peptide domain 91-96 is the active domain site, but further research will need to be conducted to confirm this conclusion.

Location

Wall 209

Start Date

3-25-2017 11:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 11:15 AM

E4 Domains and Their Anti-Fibrotic Nature

Wall 209

Scleroderma is an autoimmune disease that has a high death rate due to fibrosis. Recently a peptide called E4 has been identified to lessen the effects and even treat fibrosis. However, this peptide is difficult and costly to synthesize. Therefore, research has been conducted to identify a smaller active domain of E4 to be used in treatments. This experiment was conducted over a three week period and began with the culturing of three cells lines: NL-98, NL-88, and NL-82. After, all of the cells lines were treated with TGF and/or peptide domains and harvested into cell lysates and supernatants. The lysates were then probed for GAPDH, Collagen 1A1, MMP, and Fibronectin, while the supernatants were probed for just Collagen 1A1, MMP, and Fibronectin. The data from the Westen Blots were analyzed using densitometry. The final conclusion after looking at the resulting graphs is that peptide domain 91-96 is the active domain site, but further research will need to be conducted to confirm this conclusion.