Temporal Release Dynamics of Various Temperature Sensitive Liposome formulation

School Name

Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Mentor

Mentor: Dieter Haemmerich, Medical University of South Carolina

Abstract

Drug delivery systems have been developed to target tumors and decrease toxicity and side effects associated with chemotherapy. Temperature Sensitive Liposomes (TSLs) are a type of nanoparticle (100-200 nm) that can act as a drug delivery system. TSLs contain a drug and release it at hyperthermic temperatures (>40°C), and, with localized hyperthermia, can release its contents directly to a tumor site. Blood stays at a tumor site for only a few seconds, so the drug must be released quickly in order to be most effective. Therefore, it’s important to test and characterize various conditions for drug release from TSLs in order to find conditions that allow for the greatest drug release in the shortest time period. In this experiment, a novel setup was created to study release dynamics of doxorubicin (DOX) under varying conditions: temperature, liposome formulation, and the solution in which DOX is released. TSL-DOX was pumped through a thin capillary tube set above a Peltier element, allowing rapid heating and thereby releasing DOX from TSL. DOX fluorescence is quenched inside TSL, but once released, DOX can fluoresce and its concentration can be measured. The Peltier element was heated to temperatures ranging from 37°C to 45°C in 1°C intervals. Three formulations were tested (citrate buffer, ammonium sulfate buffer, and commercial Thermodox) in two different solutions (phosphate buffered saline and fetal bovine serum). The citrate buffer formulation of TSL-DOX had the greatest and quickest DOX-release at 45°C in FBS, at 80% DOX release within 1.5 seconds.

Location

Wall 118

Start Date

3-25-2017 8:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 8:45 AM

Temporal Release Dynamics of Various Temperature Sensitive Liposome formulation

Wall 118

Drug delivery systems have been developed to target tumors and decrease toxicity and side effects associated with chemotherapy. Temperature Sensitive Liposomes (TSLs) are a type of nanoparticle (100-200 nm) that can act as a drug delivery system. TSLs contain a drug and release it at hyperthermic temperatures (>40°C), and, with localized hyperthermia, can release its contents directly to a tumor site. Blood stays at a tumor site for only a few seconds, so the drug must be released quickly in order to be most effective. Therefore, it’s important to test and characterize various conditions for drug release from TSLs in order to find conditions that allow for the greatest drug release in the shortest time period. In this experiment, a novel setup was created to study release dynamics of doxorubicin (DOX) under varying conditions: temperature, liposome formulation, and the solution in which DOX is released. TSL-DOX was pumped through a thin capillary tube set above a Peltier element, allowing rapid heating and thereby releasing DOX from TSL. DOX fluorescence is quenched inside TSL, but once released, DOX can fluoresce and its concentration can be measured. The Peltier element was heated to temperatures ranging from 37°C to 45°C in 1°C intervals. Three formulations were tested (citrate buffer, ammonium sulfate buffer, and commercial Thermodox) in two different solutions (phosphate buffered saline and fetal bovine serum). The citrate buffer formulation of TSL-DOX had the greatest and quickest DOX-release at 45°C in FBS, at 80% DOX release within 1.5 seconds.