Exploring the Antioxidant Properties of Panax quinquefolius and its Components

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: Lorne Hofseth, University of South Carolina

Written Paper Award

1st Place

Abstract

Around 93,090 cases and 49,700 deaths, resulting from colorectal cancer, were estimated for 2015 (“Cancer facts and figures 2015,” 2015). This study focused on ulcerative colitis induced colon cancer which are promoted through inflammation. The purpose of this study is to observe what component of american ginseng allows the anti-inflammatory pathway to open up. It was hypothesized that whole ginseng would decrease the number of reactive oxygen species the most, only because hexane fraction IV and panaxynol are being used. However, only based on the extracts, the panaxynol will decrease the most reactive oxygen species and the immunofluorescence will show more nrf2 outside of the nucleus of the ANA-1 cells. The study was performed by treating ANA-1 cells with whole ginseng, hexane fraction IV, and panaxynol for 0, 1, 2, or 3 hours and then irritating them with interferon gamma for 24 hours. An immunofluorescence was then conducted to observe the location of the Nrf2 for each designated treatment. In the luminescence portion of the experiment, the trend displayed was that the whole ginseng decreased the most inflammation as the time treatment increased. However, out of the extracts, the panaxynol decreased the most inflammation as the time treatment increased. The immunofluorescence portion of the experiment showed that the Nrf2 was predominantly expressed and dispersed outside the cell for the panaxynol. The purpose of this study was to observe what component of american ginseng allows the anti-inflammatory pathway to open up.

Location

Wall 209

Start Date

3-25-2017 11:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 11:30 AM

Exploring the Antioxidant Properties of Panax quinquefolius and its Components

Wall 209

Around 93,090 cases and 49,700 deaths, resulting from colorectal cancer, were estimated for 2015 (“Cancer facts and figures 2015,” 2015). This study focused on ulcerative colitis induced colon cancer which are promoted through inflammation. The purpose of this study is to observe what component of american ginseng allows the anti-inflammatory pathway to open up. It was hypothesized that whole ginseng would decrease the number of reactive oxygen species the most, only because hexane fraction IV and panaxynol are being used. However, only based on the extracts, the panaxynol will decrease the most reactive oxygen species and the immunofluorescence will show more nrf2 outside of the nucleus of the ANA-1 cells. The study was performed by treating ANA-1 cells with whole ginseng, hexane fraction IV, and panaxynol for 0, 1, 2, or 3 hours and then irritating them with interferon gamma for 24 hours. An immunofluorescence was then conducted to observe the location of the Nrf2 for each designated treatment. In the luminescence portion of the experiment, the trend displayed was that the whole ginseng decreased the most inflammation as the time treatment increased. However, out of the extracts, the panaxynol decreased the most inflammation as the time treatment increased. The immunofluorescence portion of the experiment showed that the Nrf2 was predominantly expressed and dispersed outside the cell for the panaxynol. The purpose of this study was to observe what component of american ginseng allows the anti-inflammatory pathway to open up.