The Role of Aldehyde Dehydrogenase 2 In Liver Sichemia/Reperfusion Injury

School Name

Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Mentor

Mentor: Zhi Zhong, Medical University of South Carolina

Written Paper Award

2nd Place

Abstract

Aldehyde Dehydrogenase Activator-1 (Alda-1) is an agonist that increases the production of the enzyme Aldehyde Dehydrogenase-2 (ALDH-2). Studies have shown that ALDH-2 is helpful in degrading harmful aldehydes. With knowing this, it has become a drug of interest in liver related drug research. If ALDH-2 plays an important role in prevention of hepatic ischemia/reperfusion injury, ALDH-2 deficiency should exacerbate ischemia and activation of ALDH-2should attenuate ischemia/reperfusion injury. The aim of this research was to determine whether ischemia/reperfusion injury increased in the ALDH-2 deficient mice and also to confirm that Alda-1 has protective effects against ischemia/reperfusion injury. In our investigation, we wanted to determine if the Alda-1’s activation of ALDH-2 was causing the desired effect (to protect the liver) and not an off target effect of introducing the Alda-1. This was done by analyzing the livers of five mice: Control, Wild Type, ALDH-2 Knock Out (KO), Vehicle, and Alda-1. The ALDH-2 KO and Wild Type underwent ischemia for thirty minutes and reperfused. The Vehicle and the Alda-1 underwent ischemia for one hour and reperfused. After reperfusion the liver samples were collected six hours after the process was performed. We measured the amount of alanine transaminase (ALT), cleaved caspase 3 (CC3), Myeloperoxidase (MPO), and F4/80 through western blotting. We also investigated the histology of the cells. All of our data confirmed that ALDH-2 does have protective effects against liver ischemia/reperfusion injury. In future studies statistical analysis and a larger sample size is needed to confirm these findings.

Location

Wall 118

Start Date

3-25-2017 9:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 9:45 AM

The Role of Aldehyde Dehydrogenase 2 In Liver Sichemia/Reperfusion Injury

Wall 118

Aldehyde Dehydrogenase Activator-1 (Alda-1) is an agonist that increases the production of the enzyme Aldehyde Dehydrogenase-2 (ALDH-2). Studies have shown that ALDH-2 is helpful in degrading harmful aldehydes. With knowing this, it has become a drug of interest in liver related drug research. If ALDH-2 plays an important role in prevention of hepatic ischemia/reperfusion injury, ALDH-2 deficiency should exacerbate ischemia and activation of ALDH-2should attenuate ischemia/reperfusion injury. The aim of this research was to determine whether ischemia/reperfusion injury increased in the ALDH-2 deficient mice and also to confirm that Alda-1 has protective effects against ischemia/reperfusion injury. In our investigation, we wanted to determine if the Alda-1’s activation of ALDH-2 was causing the desired effect (to protect the liver) and not an off target effect of introducing the Alda-1. This was done by analyzing the livers of five mice: Control, Wild Type, ALDH-2 Knock Out (KO), Vehicle, and Alda-1. The ALDH-2 KO and Wild Type underwent ischemia for thirty minutes and reperfused. The Vehicle and the Alda-1 underwent ischemia for one hour and reperfused. After reperfusion the liver samples were collected six hours after the process was performed. We measured the amount of alanine transaminase (ALT), cleaved caspase 3 (CC3), Myeloperoxidase (MPO), and F4/80 through western blotting. We also investigated the histology of the cells. All of our data confirmed that ALDH-2 does have protective effects against liver ischemia/reperfusion injury. In future studies statistical analysis and a larger sample size is needed to confirm these findings.