Cloning of the Vacuolar H+ - Pyrophosphatase proton pump gene in seashore paspalum
School Name
Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Botany
Presentation Type
Mentored
Oral Presentation Award
1st Place
Abstract
The Vacuolar H+-Pyrophosphatase Proton Pump Gene is a gene that allows plants to effectively pump ions from their surroundings into their center vacuoles through the use of a very strong proton pump (Li et al, 2009). This ability to effectively pump things such as sodium, chloride, and potassium ions into its center vacuole, so that the excess of these ions cannot wreak havoc on the natural and delicate chemical processes inside a plant cell, means that plants that express this gene can survive in environments with higher salt content. If this gene can be isolated, amplified, and cloned, then it can be possible to make transgenic plants that would express this gene and be able to survive in high salt conditions in which they otherwise cannot survive. The use of multiple polymerase chain reactions with succeeding primers and nested primers allowed us to effectively amplify, and will later allow the lab to clone, the target gene in the high salt tolerant plant seashore paspalum (Paspalum vaginatum).
Recommended Citation
Crago, Nathaniel, "Cloning of the Vacuolar H+ - Pyrophosphatase proton pump gene in seashore paspalum" (2018). South Carolina Junior Academy of Science. 10.
https://scholarexchange.furman.edu/scjas/2018/all/10
Location
Neville 105
Start Date
4-14-2018 11:30 AM
Presentation Format
Oral and Written
Cloning of the Vacuolar H+ - Pyrophosphatase proton pump gene in seashore paspalum
Neville 105
The Vacuolar H+-Pyrophosphatase Proton Pump Gene is a gene that allows plants to effectively pump ions from their surroundings into their center vacuoles through the use of a very strong proton pump (Li et al, 2009). This ability to effectively pump things such as sodium, chloride, and potassium ions into its center vacuole, so that the excess of these ions cannot wreak havoc on the natural and delicate chemical processes inside a plant cell, means that plants that express this gene can survive in environments with higher salt content. If this gene can be isolated, amplified, and cloned, then it can be possible to make transgenic plants that would express this gene and be able to survive in high salt conditions in which they otherwise cannot survive. The use of multiple polymerase chain reactions with succeeding primers and nested primers allowed us to effectively amplify, and will later allow the lab to clone, the target gene in the high salt tolerant plant seashore paspalum (Paspalum vaginatum).
