Title

Cloning of the Vacuolar H+ - Pyrophosphatase proton pump gene in seashore paspalum

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Botany

Presentation Type

Mentored

Oral Presentation Award

1st Place

Abstract

The Vacuolar H+-Pyrophosphatase Proton Pump Gene is a gene that allows plants to effectively pump ions from their surroundings into their center vacuoles through the use of a very strong proton pump (Li et al, 2009). This ability to effectively pump things such as sodium, chloride, and potassium ions into its center vacuole, so that the excess of these ions cannot wreak havoc on the natural and delicate chemical processes inside a plant cell, means that plants that express this gene can survive in environments with higher salt content. If this gene can be isolated, amplified, and cloned, then it can be possible to make transgenic plants that would express this gene and be able to survive in high salt conditions in which they otherwise cannot survive. The use of multiple polymerase chain reactions with succeeding primers and nested primers allowed us to effectively amplify, and will later allow the lab to clone, the target gene in the high salt tolerant plant seashore paspalum (Paspalum vaginatum).

Location

Neville 105

Start Date

4-14-2018 11:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 11:30 AM

Cloning of the Vacuolar H+ - Pyrophosphatase proton pump gene in seashore paspalum

Neville 105

The Vacuolar H+-Pyrophosphatase Proton Pump Gene is a gene that allows plants to effectively pump ions from their surroundings into their center vacuoles through the use of a very strong proton pump (Li et al, 2009). This ability to effectively pump things such as sodium, chloride, and potassium ions into its center vacuole, so that the excess of these ions cannot wreak havoc on the natural and delicate chemical processes inside a plant cell, means that plants that express this gene can survive in environments with higher salt content. If this gene can be isolated, amplified, and cloned, then it can be possible to make transgenic plants that would express this gene and be able to survive in high salt conditions in which they otherwise cannot survive. The use of multiple polymerase chain reactions with succeeding primers and nested primers allowed us to effectively amplify, and will later allow the lab to clone, the target gene in the high salt tolerant plant seashore paspalum (Paspalum vaginatum).