Does the inflammatory compound DOSS promote Cancer Stem Cells expansion?
School Name
Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
Research has shown that chronic inflammation regulates the development and function of cancer stem cells(CSCs) . Docusate or dioctyl sodium sulfosuccinate (DOSS), drives both COX-2 and PPARβ in the inflammatory pathway. It was inferred that because of the link of chronic inflammation in both DOSS and cancer stem cells, DOSS promotes CSCs expansion. The goal is to see if there is a link between DOSS and CSCs expansion. To test the effect of DOSS on CSCs expansion, vector stem cells were plated into wells and treated with four conditions of DOSS. The growth of single cells was measured by an software analyze NIH ImageJ and was read by an Synergy H1 Hybrid Reader. The results showed a classic dose responsiveness with the highest concentration of DOSS, having the highest number of single cells.
Recommended Citation
Dantzler, Danielle, "Does the inflammatory compound DOSS promote Cancer Stem Cells expansion?" (2018). South Carolina Junior Academy of Science. 16.
https://scholarexchange.furman.edu/scjas/2018/all/16
Location
Neville theater
Start Date
4-14-2018 8:30 AM
Presentation Format
Oral and Written
Does the inflammatory compound DOSS promote Cancer Stem Cells expansion?
Neville theater
Research has shown that chronic inflammation regulates the development and function of cancer stem cells(CSCs) . Docusate or dioctyl sodium sulfosuccinate (DOSS), drives both COX-2 and PPARβ in the inflammatory pathway. It was inferred that because of the link of chronic inflammation in both DOSS and cancer stem cells, DOSS promotes CSCs expansion. The goal is to see if there is a link between DOSS and CSCs expansion. To test the effect of DOSS on CSCs expansion, vector stem cells were plated into wells and treated with four conditions of DOSS. The growth of single cells was measured by an software analyze NIH ImageJ and was read by an Synergy H1 Hybrid Reader. The results showed a classic dose responsiveness with the highest concentration of DOSS, having the highest number of single cells.
