Title

Does the inflammatory compound DOSS promote Cancer Stem Cells expansion?

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

Research has shown that chronic inflammation regulates the development and function of cancer stem cells(CSCs) . Docusate or dioctyl sodium sulfosuccinate (DOSS), drives both COX-2 and PPARβ in the inflammatory pathway. It was inferred that because of the link of chronic inflammation in both DOSS and cancer stem cells, DOSS promotes CSCs expansion. The goal is to see if there is a link between DOSS and CSCs expansion. To test the effect of DOSS on CSCs expansion, vector stem cells were plated into wells and treated with four conditions of DOSS. The growth of single cells was measured by an software analyze NIH ImageJ and was read by an Synergy H1 Hybrid Reader. The results showed a classic dose responsiveness with the highest concentration of DOSS, having the highest number of single cells.

Location

Neville theater

Start Date

4-14-2018 8:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 8:30 AM

Does the inflammatory compound DOSS promote Cancer Stem Cells expansion?

Neville theater

Research has shown that chronic inflammation regulates the development and function of cancer stem cells(CSCs) . Docusate or dioctyl sodium sulfosuccinate (DOSS), drives both COX-2 and PPARβ in the inflammatory pathway. It was inferred that because of the link of chronic inflammation in both DOSS and cancer stem cells, DOSS promotes CSCs expansion. The goal is to see if there is a link between DOSS and CSCs expansion. To test the effect of DOSS on CSCs expansion, vector stem cells were plated into wells and treated with four conditions of DOSS. The growth of single cells was measured by an software analyze NIH ImageJ and was read by an Synergy H1 Hybrid Reader. The results showed a classic dose responsiveness with the highest concentration of DOSS, having the highest number of single cells.