The effect of Copper(II) sulfate pentahydrate on abscisic acid in Cucurbita pepo

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Environmental Science

Presentation Type

Non-Mentored

Abstract

The purpose of this experiment was to determine whether copper (II) sulfate pentahydrate can positively or negatively affect the amount of abscisic acid (ABA), represented by parts per million (ppm), produced by Cucurbita pepo plants. If the copper (II) sulfate pentahydrate were to positively affect the amount of ABA, the plants would undergo fermentation quickly and have a more vibrant color to the leafs and stem. It was hypothesized that the copper (II) sulfate pentahydrate would cause the zucchini plant to produce less ABA, thus causing poor physical characteristics of the plants, such as poor color and poor growth. There were 2 groups in this experiment consisting of 30 zucchini plants, a control group and an experimental group. The control group was given only water and sunlight everyday until the plants finished fermentation. The experimental group was given the same amount of sunlight but 26% concentrated copper (II) sulfate pentahydrate everyday. Both groups had the same amount and type of soil. After the control group plants finished fermentation, the ABA was measured using an electrochemical ABA sensor, also referred as an ABA meter, obtained from Amazon.com. This measures the amount of ABA produced by each plant. Each plant was separated from the rest. The unpooled independent t-test, represented by graph 4, shows the statistical significance of this experiment, having an low p-value. The control group (M=0.75, SD=0.12) reported significantly higher levels of ABA ppm than the experimental group (M=0.12, SD=0.06), t(29) = 23.90, p < 0.0001.

Location

Lassiter 220

Start Date

4-14-2018 11:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 11:30 AM

The effect of Copper(II) sulfate pentahydrate on abscisic acid in Cucurbita pepo

Lassiter 220

The purpose of this experiment was to determine whether copper (II) sulfate pentahydrate can positively or negatively affect the amount of abscisic acid (ABA), represented by parts per million (ppm), produced by Cucurbita pepo plants. If the copper (II) sulfate pentahydrate were to positively affect the amount of ABA, the plants would undergo fermentation quickly and have a more vibrant color to the leafs and stem. It was hypothesized that the copper (II) sulfate pentahydrate would cause the zucchini plant to produce less ABA, thus causing poor physical characteristics of the plants, such as poor color and poor growth. There were 2 groups in this experiment consisting of 30 zucchini plants, a control group and an experimental group. The control group was given only water and sunlight everyday until the plants finished fermentation. The experimental group was given the same amount of sunlight but 26% concentrated copper (II) sulfate pentahydrate everyday. Both groups had the same amount and type of soil. After the control group plants finished fermentation, the ABA was measured using an electrochemical ABA sensor, also referred as an ABA meter, obtained from Amazon.com. This measures the amount of ABA produced by each plant. Each plant was separated from the rest. The unpooled independent t-test, represented by graph 4, shows the statistical significance of this experiment, having an low p-value. The control group (M=0.75, SD=0.12) reported significantly higher levels of ABA ppm than the experimental group (M=0.12, SD=0.06), t(29) = 23.90, p < 0.0001.