Effect of Benzyl Salicylate on Neuronal Cell Lines 2266 and 2267
School Name
Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Physiology and Health
Presentation Type
Mentored
Oral Presentation Award
2nd Place
Abstract
Autism Spectrum Disorder (ASD) is the name of a group of pervasive, early childhood, neuronal developmental disorders that affect infants all the way into adulthood. Children and adults affected with ASD have a wide range of symptoms with different levels of disabilities. The causes of ASD are unknown, however, research suggests that both genes and the environment play important roles. A study examined toxicant exposure during the prenatal period in parents of children with ASD and reported a potential association between toxicant exposures in the prenatal period and autism risk. In this research, Benzyl Salicylate was tested for effects on neuronal morphology. Two neuronal cell lines: 2266 (Female) and 2267 (Male) were cultured for a week and then divided into an 8-well chamber. The two cell lines treated with concentrations 15.625 ng/mL, 31.25 ng/ mL, and 62.5 ng/mL of Benzyl Salicylate for 48 hours. They were then stained with Eosin and Hematoxylin and observed under a microscope for morphological changes of the neurons such as axon degeneration, axonal elongation, syncytia formation, and chromatolysis. In comparison to the control, benzyl salicylate did not significantly affect the two neuronal cell lines. Further research will need to be done to confirm these results.
Recommended Citation
Aduma, Calvin, "Effect of Benzyl Salicylate on Neuronal Cell Lines 2266 and 2267" (2018). South Carolina Junior Academy of Science. 76.
https://scholarexchange.furman.edu/scjas/2018/all/76
Location
Neville 322
Start Date
4-14-2018 10:45 AM
Presentation Format
Oral and Written
Effect of Benzyl Salicylate on Neuronal Cell Lines 2266 and 2267
Neville 322
Autism Spectrum Disorder (ASD) is the name of a group of pervasive, early childhood, neuronal developmental disorders that affect infants all the way into adulthood. Children and adults affected with ASD have a wide range of symptoms with different levels of disabilities. The causes of ASD are unknown, however, research suggests that both genes and the environment play important roles. A study examined toxicant exposure during the prenatal period in parents of children with ASD and reported a potential association between toxicant exposures in the prenatal period and autism risk. In this research, Benzyl Salicylate was tested for effects on neuronal morphology. Two neuronal cell lines: 2266 (Female) and 2267 (Male) were cultured for a week and then divided into an 8-well chamber. The two cell lines treated with concentrations 15.625 ng/mL, 31.25 ng/ mL, and 62.5 ng/mL of Benzyl Salicylate for 48 hours. They were then stained with Eosin and Hematoxylin and observed under a microscope for morphological changes of the neurons such as axon degeneration, axonal elongation, syncytia formation, and chromatolysis. In comparison to the control, benzyl salicylate did not significantly affect the two neuronal cell lines. Further research will need to be done to confirm these results.
