Title

Effect of Benzyl Salicylate on Neuronal Cell Lines 2266 and 2267

Author(s)

Calvin Aduma, GSSM

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Oral Presentation Award

2nd Place

Abstract

Autism Spectrum Disorder (ASD) is the name of a group of pervasive, early childhood, neuronal developmental disorders that affect infants all the way into adulthood. Children and adults affected with ASD have a wide range of symptoms with different levels of disabilities. The causes of ASD are unknown, however, research suggests that both genes and the environment play important roles. A study examined toxicant exposure during the prenatal period in parents of children with ASD and reported a potential association between toxicant exposures in the prenatal period and autism risk. In this research, Benzyl Salicylate was tested for effects on neuronal morphology. Two neuronal cell lines: 2266 (Female) and 2267 (Male) were cultured for a week and then divided into an 8-well chamber. The two cell lines treated with concentrations 15.625 ng/mL, 31.25 ng/ mL, and 62.5 ng/mL of Benzyl Salicylate for 48 hours. They were then stained with Eosin and Hematoxylin and observed under a microscope for morphological changes of the neurons such as axon degeneration, axonal elongation, syncytia formation, and chromatolysis. In comparison to the control, benzyl salicylate did not significantly affect the two neuronal cell lines. Further research will need to be done to confirm these results.

Location

Neville 322

Start Date

4-14-2018 10:45 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 10:45 AM

Effect of Benzyl Salicylate on Neuronal Cell Lines 2266 and 2267

Neville 322

Autism Spectrum Disorder (ASD) is the name of a group of pervasive, early childhood, neuronal developmental disorders that affect infants all the way into adulthood. Children and adults affected with ASD have a wide range of symptoms with different levels of disabilities. The causes of ASD are unknown, however, research suggests that both genes and the environment play important roles. A study examined toxicant exposure during the prenatal period in parents of children with ASD and reported a potential association between toxicant exposures in the prenatal period and autism risk. In this research, Benzyl Salicylate was tested for effects on neuronal morphology. Two neuronal cell lines: 2266 (Female) and 2267 (Male) were cultured for a week and then divided into an 8-well chamber. The two cell lines treated with concentrations 15.625 ng/mL, 31.25 ng/ mL, and 62.5 ng/mL of Benzyl Salicylate for 48 hours. They were then stained with Eosin and Hematoxylin and observed under a microscope for morphological changes of the neurons such as axon degeneration, axonal elongation, syncytia formation, and chromatolysis. In comparison to the control, benzyl salicylate did not significantly affect the two neuronal cell lines. Further research will need to be done to confirm these results.