Mitigating the Immune Response of Transplant Tissues
School Name
Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Physiology and Health
Presentation Type
Mentored
Written Paper Award
3rd Place
Abstract
Organ transplantation is one of the foremost branches of surgery today. Hundreds of thousands of patients are in need of an organ transplant. However, large amounts of transplant organs are being discarded due to short preservation times. Ice-free cryopreservation is a method that is more cost-effective and has a lower risk of damage to the tissues than conventionally frozen cryopreservation. We tested the ice-free cryopreservation method, hypothesizing that it allows for storage while mitigating the immune response of the transplant. Fresh, nonpreserved porcine arteries were compared with ice-free cryopreserved arteries through histopathology, immunochemistry, and MALDI Imaging Mass Spectrometry. The arteries were examined for N-linked glycan signatures correlating to inflammation and an immune response. In the preliminary results of the study, 60 N-linked glycans have been mapped across the arterial tissues. Some of these N-linked glycans have shown significantly greater presences in arteries expected to have increased immune responses. Matching the MALDI images with the immunohistochemistry stains will allow us to determine which N-linked glycans are correlated to inflammation. We can use the intensities of the N-linked glycan signatures to quantify the immune response in each of the arteries. Then, we will then be able to determine whether ice-free cryopreservation allows storage while mitigating the immune response of the transplant.
Recommended Citation
Hamid, Rasikh, "Mitigating the Immune Response of Transplant Tissues" (2018). South Carolina Junior Academy of Science. 79.
https://scholarexchange.furman.edu/scjas/2018/all/79
Location
Neville 322
Start Date
4-14-2018 11:15 AM
Presentation Format
Oral and Written
Mitigating the Immune Response of Transplant Tissues
Neville 322
Organ transplantation is one of the foremost branches of surgery today. Hundreds of thousands of patients are in need of an organ transplant. However, large amounts of transplant organs are being discarded due to short preservation times. Ice-free cryopreservation is a method that is more cost-effective and has a lower risk of damage to the tissues than conventionally frozen cryopreservation. We tested the ice-free cryopreservation method, hypothesizing that it allows for storage while mitigating the immune response of the transplant. Fresh, nonpreserved porcine arteries were compared with ice-free cryopreserved arteries through histopathology, immunochemistry, and MALDI Imaging Mass Spectrometry. The arteries were examined for N-linked glycan signatures correlating to inflammation and an immune response. In the preliminary results of the study, 60 N-linked glycans have been mapped across the arterial tissues. Some of these N-linked glycans have shown significantly greater presences in arteries expected to have increased immune responses. Matching the MALDI images with the immunohistochemistry stains will allow us to determine which N-linked glycans are correlated to inflammation. We can use the intensities of the N-linked glycan signatures to quantify the immune response in each of the arteries. Then, we will then be able to determine whether ice-free cryopreservation allows storage while mitigating the immune response of the transplant.
