Histological Analysis of the Osteoarthritic Knee Joint In DHGPS Treated With MSCS

Heather CoshFollow

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Oral Presentation Award

1st Place

Abstract

Osteoarthritis (OA) results in the degradation of the joint, affecting the articular cartilage, subchondral bone, and surrounding synovium. OA affects nearly 34% of the world’s population. Currently, no cure for OA exists considering treatment options only provide palliative care; therefore, it is desirable to investigate other regenerative strategies to mitigate the progression of this debilitating disease. The study objective was to investigate the therapeutic efficacy of human amnion derived stem cells (hAMSC) and adipose derived stem cells (hADSC) when intra-articularly injected in Dunkin Hartley Guinea Pig (DHGP) knees. A therapeutic treatment was injected into the left knee and a saline group was injected into the right knee of the DHGPs. The treatment options included Hyaluronic Acid (HA), HA+hAMSC, and HA+hADSC. After three injections over the span of six months, the knees were harvested for analysis. Twenty-four samples were formalin fixed in 10% formic acid and decalcified in Immunocal solution. The samples were then embedded in paraffin wax for sectioning. The microarchitecture of the knee tissue was analyzed using a universal histological grading scale provided by OARSI, in which increasing score represents further degradation of articular cartilage and proteoglycan content. Immunohistochemistry (IHC) was also performed to examine macrophage infiltration of the synovium using a mouse anti-guinea pig primary antibody accompanied with an anti-mouse IgG secondary antibody. Histological results showed that treatment groups had significantly lower scores than with their corresponding saline controls, ultimately showing that treatment with mesenchymal stem cell (MSC) sources may help to mitigate the progression of OA.

Location

Founders Hall 142 A

Start Date

3-30-2019 10:15 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 10:15 AM

Histological Analysis of the Osteoarthritic Knee Joint In DHGPS Treated With MSCS

Founders Hall 142 A

Osteoarthritis (OA) results in the degradation of the joint, affecting the articular cartilage, subchondral bone, and surrounding synovium. OA affects nearly 34% of the world’s population. Currently, no cure for OA exists considering treatment options only provide palliative care; therefore, it is desirable to investigate other regenerative strategies to mitigate the progression of this debilitating disease. The study objective was to investigate the therapeutic efficacy of human amnion derived stem cells (hAMSC) and adipose derived stem cells (hADSC) when intra-articularly injected in Dunkin Hartley Guinea Pig (DHGP) knees. A therapeutic treatment was injected into the left knee and a saline group was injected into the right knee of the DHGPs. The treatment options included Hyaluronic Acid (HA), HA+hAMSC, and HA+hADSC. After three injections over the span of six months, the knees were harvested for analysis. Twenty-four samples were formalin fixed in 10% formic acid and decalcified in Immunocal solution. The samples were then embedded in paraffin wax for sectioning. The microarchitecture of the knee tissue was analyzed using a universal histological grading scale provided by OARSI, in which increasing score represents further degradation of articular cartilage and proteoglycan content. Immunohistochemistry (IHC) was also performed to examine macrophage infiltration of the synovium using a mouse anti-guinea pig primary antibody accompanied with an anti-mouse IgG secondary antibody. Histological results showed that treatment groups had significantly lower scores than with their corresponding saline controls, ultimately showing that treatment with mesenchymal stem cell (MSC) sources may help to mitigate the progression of OA.