The Antibacterial Effect of Cotton Infused With Colloidal Silver and Cotton Infused with Colloidal Copper on Non-Virulent Strains of E. Coli Over Short Periods of Time

Author(s)

Yash PatelFollow

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Written Paper Award

2nd Place

Abstract

In recent years there has been a rise in hospital acquired infections which are very prevalent because they consist of multidrug resistant bacteria, and biofilms. The purpose of this study was to see if silver and copper nanoparticles are effective against household bacteria, such as E. coli K-12. It was hypothesised that the silver colloid would perform better then both the copper colloid and the Lysol disinfectant. It was further hypothesised that the copper colloid would perform better than the Lysol disinfectant. Cheesecloth was used to simulate the wiping effect that would occur if it were to be used as a antibacterial spray. The cheesecloth, saturated with either colloidal copper, colloidal silver, or Lysol disinfectant, was used as Kirby-Bauer Disks to conduct a Kirby-Bauer Disk Diffusion Test. The zone of inhibition area per centimeter squared of cheesecloth was used to conduct an ANOVA test, at alpha equal to 0.05. The ANOVA test showed, [F(2,86)=0.42, p=0.662], that there was no significant difference between the zones of inhibition per area of cheesecloth for lysol, copper colloid, and silver colloid. The result of this study showed that colloidal copper and colloidal silver should not be primarily used for household bacteria such as E. coli K-12 because they perform at the same level as regular disinfectants, such as Lysol.

Location

Founders Hall 216 B

Start Date

3-30-2019 9:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 9:30 AM

The Antibacterial Effect of Cotton Infused With Colloidal Silver and Cotton Infused with Colloidal Copper on Non-Virulent Strains of E. Coli Over Short Periods of Time

Founders Hall 216 B

In recent years there has been a rise in hospital acquired infections which are very prevalent because they consist of multidrug resistant bacteria, and biofilms. The purpose of this study was to see if silver and copper nanoparticles are effective against household bacteria, such as E. coli K-12. It was hypothesised that the silver colloid would perform better then both the copper colloid and the Lysol disinfectant. It was further hypothesised that the copper colloid would perform better than the Lysol disinfectant. Cheesecloth was used to simulate the wiping effect that would occur if it were to be used as a antibacterial spray. The cheesecloth, saturated with either colloidal copper, colloidal silver, or Lysol disinfectant, was used as Kirby-Bauer Disks to conduct a Kirby-Bauer Disk Diffusion Test. The zone of inhibition area per centimeter squared of cheesecloth was used to conduct an ANOVA test, at alpha equal to 0.05. The ANOVA test showed, [F(2,86)=0.42, p=0.662], that there was no significant difference between the zones of inhibition per area of cheesecloth for lysol, copper colloid, and silver colloid. The result of this study showed that colloidal copper and colloidal silver should not be primarily used for household bacteria such as E. coli K-12 because they perform at the same level as regular disinfectants, such as Lysol.