The Effect of Chaenomeles Speciosa (DRI) on the Response to thermotaxis in Caenorhabditis Elegans Exposed to Ethanol During Development
School Name
Spring Valley High School
Grade Level
11th Grade
Presentation Topic
Physiology and Health
Presentation Type
Non-Mentored
Oral Presentation Award
2nd Place
Written Paper Award
4th Place
Abstract
Fetal alcohol syndrome (FAS) is a disorder that results from the consumption of alcohol by pregnant women. Certain substances such as DRIs may help prevent the onset of symptoms including, poor intrauterine growth and delayed developmental milestones. C. elegans is an excellent model for FAS as there are many stimuli that can be tested to show changes in neurological capability. The purpose of this experiment was to test the efficiency of Chaenomeles Speciosa (DRI) against FAS modeled by C. elegans. It was hypothesized that highest amount of Chaenomeles Speciosa would allow for the highest percentage of C. elegans in the coldest section of the thermotaxis gradient. For experimentation, 50 square petri dishes, divided into 4 sections, were seeded with C. elegans. During this time, 30 microliters of ethanol and varying amounts of treatment was added. After 72 hours, the petri dishes were then placed on a thermotaxis gradient, with temperature increasing from one side to the other, and the number of C. elegans in each section was recorded after 30 minutes. However, percentages were used as results because there were a different number of C. elegans that matured in each petri dish. A percentage would yield a quantification that would fit all petri dishes. The p-value for the percentage and treatment showed statistically insignificant results with p=0.987. A two-way ANOVA was conducted at ɑ=0.01.The p-value for the percentage and section number showed statistically significant results for that with it being 0.000026. Regardless, flowering quince appeared to be an effective treatment in combating FAS.
Recommended Citation
Kerai, Deiveek, "The Effect of Chaenomeles Speciosa (DRI) on the Response to thermotaxis in Caenorhabditis Elegans Exposed to Ethanol During Development" (2019). South Carolina Junior Academy of Science. 184.
https://scholarexchange.furman.edu/scjas/2019/all/184
Location
Founders Hall 142 B
Start Date
3-30-2019 9:45 AM
Presentation Format
Oral and Written
Group Project
No
The Effect of Chaenomeles Speciosa (DRI) on the Response to thermotaxis in Caenorhabditis Elegans Exposed to Ethanol During Development
Founders Hall 142 B
Fetal alcohol syndrome (FAS) is a disorder that results from the consumption of alcohol by pregnant women. Certain substances such as DRIs may help prevent the onset of symptoms including, poor intrauterine growth and delayed developmental milestones. C. elegans is an excellent model for FAS as there are many stimuli that can be tested to show changes in neurological capability. The purpose of this experiment was to test the efficiency of Chaenomeles Speciosa (DRI) against FAS modeled by C. elegans. It was hypothesized that highest amount of Chaenomeles Speciosa would allow for the highest percentage of C. elegans in the coldest section of the thermotaxis gradient. For experimentation, 50 square petri dishes, divided into 4 sections, were seeded with C. elegans. During this time, 30 microliters of ethanol and varying amounts of treatment was added. After 72 hours, the petri dishes were then placed on a thermotaxis gradient, with temperature increasing from one side to the other, and the number of C. elegans in each section was recorded after 30 minutes. However, percentages were used as results because there were a different number of C. elegans that matured in each petri dish. A percentage would yield a quantification that would fit all petri dishes. The p-value for the percentage and treatment showed statistically insignificant results with p=0.987. A two-way ANOVA was conducted at ɑ=0.01.The p-value for the percentage and section number showed statistically significant results for that with it being 0.000026. Regardless, flowering quince appeared to be an effective treatment in combating FAS.