The Effect of Chaenomeles Speciosa (DRI) on the Response to thermotaxis in Caenorhabditis Elegans Exposed to Ethanol During Development

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Oral Presentation Award

2nd Place

Written Paper Award

4th Place

Abstract

Fetal alcohol syndrome (FAS) is a disorder that results from the consumption of alcohol by pregnant women. Certain substances such as DRIs may help prevent the onset of symptoms including, poor intrauterine growth and delayed developmental milestones. C. elegans is an excellent model for FAS as there are many stimuli that can be tested to show changes in neurological capability. The purpose of this experiment was to test the efficiency of Chaenomeles Speciosa (DRI) against FAS modeled by C. elegans. It was hypothesized that highest amount of Chaenomeles Speciosa would allow for the highest percentage of C. elegans in the coldest section of the thermotaxis gradient. For experimentation, 50 square petri dishes, divided into 4 sections, were seeded with C. elegans. During this time, 30 microliters of ethanol and varying amounts of treatment was added. After 72 hours, the petri dishes were then placed on a thermotaxis gradient, with temperature increasing from one side to the other, and the number of C. elegans in each section was recorded after 30 minutes. However, percentages were used as results because there were a different number of C. elegans that matured in each petri dish. A percentage would yield a quantification that would fit all petri dishes. The p-value for the percentage and treatment showed statistically insignificant results with p=0.987. A two-way ANOVA was conducted at ɑ=0.01.The p-value for the percentage and section number showed statistically significant results for that with it being 0.000026. Regardless, flowering quince appeared to be an effective treatment in combating FAS.

Location

Founders Hall 142 B

Start Date

3-30-2019 9:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 9:45 AM

The Effect of Chaenomeles Speciosa (DRI) on the Response to thermotaxis in Caenorhabditis Elegans Exposed to Ethanol During Development

Founders Hall 142 B

Fetal alcohol syndrome (FAS) is a disorder that results from the consumption of alcohol by pregnant women. Certain substances such as DRIs may help prevent the onset of symptoms including, poor intrauterine growth and delayed developmental milestones. C. elegans is an excellent model for FAS as there are many stimuli that can be tested to show changes in neurological capability. The purpose of this experiment was to test the efficiency of Chaenomeles Speciosa (DRI) against FAS modeled by C. elegans. It was hypothesized that highest amount of Chaenomeles Speciosa would allow for the highest percentage of C. elegans in the coldest section of the thermotaxis gradient. For experimentation, 50 square petri dishes, divided into 4 sections, were seeded with C. elegans. During this time, 30 microliters of ethanol and varying amounts of treatment was added. After 72 hours, the petri dishes were then placed on a thermotaxis gradient, with temperature increasing from one side to the other, and the number of C. elegans in each section was recorded after 30 minutes. However, percentages were used as results because there were a different number of C. elegans that matured in each petri dish. A percentage would yield a quantification that would fit all petri dishes. The p-value for the percentage and treatment showed statistically insignificant results with p=0.987. A two-way ANOVA was conducted at ɑ=0.01.The p-value for the percentage and section number showed statistically significant results for that with it being 0.000026. Regardless, flowering quince appeared to be an effective treatment in combating FAS.