The Effects of Modified Resveratrol on LN229 and U87 Glioblastoma Cell Death
Abstract
Glioblastoma is one of the deadliest forms of brain cancer, and it is difficult to treat because of the blood-brain barrier. Resveratrol (RSV) is a natural, anti-inflammatory compound found in grapes and fungi, and has been shown to have detrimental effects on cancer cell lines and to be able to cross the blood brain barrier, but is also known to degrade quickly. Therefore, the aim of this research is to create a stable chemically modified resveratrol (R6A) by adding 6 carbons to the non-functional end of the molecule. R6A was tested on two glioblastoma cell lines LN229 and U87 cells. These cells were treated with various concentrations of R6A (0-100 µM) in a 96 well plate. The cells were retreated with fresh R6A every 24 hours, and an MTT assay was performed after 48 and 72 hours to check for cell death. The results indicate that LN229 cells did not show cell death at 48 hours but did show significant cell death at concentrations of 1 µM and higher after 72 hours, as compared to untreated LN229 cells. U87 cells showed that after 48 hours, there was an increase in cell death as the concentration of R6A increased. At 72 hours, there was cell death at concentrations of 1 µM and above, as compared to the control. In the future, a wider range of concentrations and time intervals should be tested. In addition, the effects of R6A should be compared with those of other RSV analogs, including RSV itself.
The Effects of Modified Resveratrol on LN229 and U87 Glioblastoma Cell Death
Founders Hall 114 A
Glioblastoma is one of the deadliest forms of brain cancer, and it is difficult to treat because of the blood-brain barrier. Resveratrol (RSV) is a natural, anti-inflammatory compound found in grapes and fungi, and has been shown to have detrimental effects on cancer cell lines and to be able to cross the blood brain barrier, but is also known to degrade quickly. Therefore, the aim of this research is to create a stable chemically modified resveratrol (R6A) by adding 6 carbons to the non-functional end of the molecule. R6A was tested on two glioblastoma cell lines LN229 and U87 cells. These cells were treated with various concentrations of R6A (0-100 µM) in a 96 well plate. The cells were retreated with fresh R6A every 24 hours, and an MTT assay was performed after 48 and 72 hours to check for cell death. The results indicate that LN229 cells did not show cell death at 48 hours but did show significant cell death at concentrations of 1 µM and higher after 72 hours, as compared to untreated LN229 cells. U87 cells showed that after 48 hours, there was an increase in cell death as the concentration of R6A increased. At 72 hours, there was cell death at concentrations of 1 µM and above, as compared to the control. In the future, a wider range of concentrations and time intervals should be tested. In addition, the effects of R6A should be compared with those of other RSV analogs, including RSV itself.
