2,2',3,5',6-Pentachlorobiphenyl (PCB-95) Induced toxicity on Early Development of Zebrafish (Danio Rerio)

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Environmental Science

Presentation Type

Mentored

Abstract

Polychlorinated Biphenyls (PCBs) are toxic chemicals that are of public and wildlife health concern because of their persistence and bio-accumulative properties. Out of 209 different congeners of PCBs, PCB -95 was used in the current study. This study serves to show that exposure to PCB-95 causes brain cell necrosis, which is one mechanism for the PCB-95 toxicity. Zebrafish (Danio rerio) embryos at 2 cell stage with intact chorion were exposed to different PCB-95 concentrations (0.25ppm, 0.5ppm,0.75ppm, 1 ppm) with two controls (E2 solution and a DMSO solution). After exposure, the embryos were incubated for 7 days. At day 7, brains were sectioned and stained with toluidine blue. Higher brain cell necrosis was observed in the treated group compared to the controls. Further, necrosis increased in the treated groups in a dose dependent manner. Together with, survival rates, morphological deformations and brain necrosis, we confirm PCB -95 exerts toxic effects on early development of an organism. The results of the present study can be coupled with molecular and other toxicological assays to reveal the possible toxic mechanisms of PCB -95 on neurogenesis of an organism.

Location

Founders Hall 213 B

Start Date

3-30-2019 9:15 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 9:15 AM

2,2',3,5',6-Pentachlorobiphenyl (PCB-95) Induced toxicity on Early Development of Zebrafish (Danio Rerio)

Founders Hall 213 B

Polychlorinated Biphenyls (PCBs) are toxic chemicals that are of public and wildlife health concern because of their persistence and bio-accumulative properties. Out of 209 different congeners of PCBs, PCB -95 was used in the current study. This study serves to show that exposure to PCB-95 causes brain cell necrosis, which is one mechanism for the PCB-95 toxicity. Zebrafish (Danio rerio) embryos at 2 cell stage with intact chorion were exposed to different PCB-95 concentrations (0.25ppm, 0.5ppm,0.75ppm, 1 ppm) with two controls (E2 solution and a DMSO solution). After exposure, the embryos were incubated for 7 days. At day 7, brains were sectioned and stained with toluidine blue. Higher brain cell necrosis was observed in the treated group compared to the controls. Further, necrosis increased in the treated groups in a dose dependent manner. Together with, survival rates, morphological deformations and brain necrosis, we confirm PCB -95 exerts toxic effects on early development of an organism. The results of the present study can be coupled with molecular and other toxicological assays to reveal the possible toxic mechanisms of PCB -95 on neurogenesis of an organism.