Title

Calcium Overload and Reactive Oxygen Species Effect on HDAC Protein Expression

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

The purpose of my research was to determine the correlation between HDAC increasing as the amount of calcium or Reactive Oxidative Species increased as well since we already know that ROS and calcium cause hearing loss and HDAC is a protein responsible for cell death. This research is important because we might be able to use HDAC inhibitors to prevent the hearing loss. I hypothesized that if more HDAC proteins are expressed by treatment with ROS or Calcium than it indicates that the HDAC proteins are caused by the increase in ROS and Calcium Influx. Some of the methods we used to get our results were western blots and cell cultures. We also treated the cells with Ionophore to represent the calcium and H2O2 to represent the ROS. Our conclusions were that increased HDACs may be caused by the Calcium Overload. The data for ROS wasn't able to be completed so we can't draw conclusions from that experiment. We need to do further testing to confirm the results. This data is important because it shows that there is some correlation between HDAC protein concentration and calcium overload which means that HDAC proteins could be a cause for hearing loss.

Location

Furman Hall 107

Start Date

3-28-2020 11:00 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 28th, 11:00 AM

Calcium Overload and Reactive Oxygen Species Effect on HDAC Protein Expression

Furman Hall 107

The purpose of my research was to determine the correlation between HDAC increasing as the amount of calcium or Reactive Oxidative Species increased as well since we already know that ROS and calcium cause hearing loss and HDAC is a protein responsible for cell death. This research is important because we might be able to use HDAC inhibitors to prevent the hearing loss. I hypothesized that if more HDAC proteins are expressed by treatment with ROS or Calcium than it indicates that the HDAC proteins are caused by the increase in ROS and Calcium Influx. Some of the methods we used to get our results were western blots and cell cultures. We also treated the cells with Ionophore to represent the calcium and H2O2 to represent the ROS. Our conclusions were that increased HDACs may be caused by the Calcium Overload. The data for ROS wasn't able to be completed so we can't draw conclusions from that experiment. We need to do further testing to confirm the results. This data is important because it shows that there is some correlation between HDAC protein concentration and calcium overload which means that HDAC proteins could be a cause for hearing loss.