Calcium Overload and Reactive Oxygen Species Effect on HDAC Protein Expression
School Name
South Carolina Governor's School for Science & Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
The purpose of my research was to determine the correlation between HDAC increasing as the amount of calcium or Reactive Oxidative Species increased as well since we already know that ROS and calcium cause hearing loss and HDAC is a protein responsible for cell death. This research is important because we might be able to use HDAC inhibitors to prevent the hearing loss. I hypothesized that if more HDAC proteins are expressed by treatment with ROS or Calcium than it indicates that the HDAC proteins are caused by the increase in ROS and Calcium Influx. Some of the methods we used to get our results were western blots and cell cultures. We also treated the cells with Ionophore to represent the calcium and H2O2 to represent the ROS. Our conclusions were that increased HDACs may be caused by the Calcium Overload. The data for ROS wasn't able to be completed so we can't draw conclusions from that experiment. We need to do further testing to confirm the results. This data is important because it shows that there is some correlation between HDAC protein concentration and calcium overload which means that HDAC proteins could be a cause for hearing loss.
Recommended Citation
Karamched, Kaushik, "Calcium Overload and Reactive Oxygen Species Effect on HDAC Protein Expression" (2020). South Carolina Junior Academy of Science. 208.
https://scholarexchange.furman.edu/scjas/2020/all/208
Location
Furman Hall 107
Start Date
3-28-2020 11:00 AM
Presentation Format
Oral Only
Group Project
No
Calcium Overload and Reactive Oxygen Species Effect on HDAC Protein Expression
Furman Hall 107
The purpose of my research was to determine the correlation between HDAC increasing as the amount of calcium or Reactive Oxidative Species increased as well since we already know that ROS and calcium cause hearing loss and HDAC is a protein responsible for cell death. This research is important because we might be able to use HDAC inhibitors to prevent the hearing loss. I hypothesized that if more HDAC proteins are expressed by treatment with ROS or Calcium than it indicates that the HDAC proteins are caused by the increase in ROS and Calcium Influx. Some of the methods we used to get our results were western blots and cell cultures. We also treated the cells with Ionophore to represent the calcium and H2O2 to represent the ROS. Our conclusions were that increased HDACs may be caused by the Calcium Overload. The data for ROS wasn't able to be completed so we can't draw conclusions from that experiment. We need to do further testing to confirm the results. This data is important because it shows that there is some correlation between HDAC protein concentration and calcium overload which means that HDAC proteins could be a cause for hearing loss.
