Title

Impact of Resveratrol on the Thigmotaxis and β-amyloid Toxicity of Caenorhabditis elegans Strain Cl4176 for Diabetes and Alzheimer's Disease Applications

Author(s)

Parth Patel

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Abstract

Alzheimer's disease and diabetes are two of the most impactful diseases in the world, projected to be the sixth and seventh leading cause of death in the United States, respectively. The purpose of this experiment was to test resveratrol, a polyphenol, as a moderator of Alzheimer's. It was hypothesized that an increased concentration of resveratrol would increase thigmotaxis response and slow β-amyloid toxicity progression for C. elegans strain cl4176 in normal conditions and diabetic conditions (glucose toxicity). A trans-resveratrol supplement was obtained, and agar plates were set up with nematode growth agar. L4 stage C. elegans were used for each test. Resveratrol was given in concentrations of 0g, 2.5g, 5g, and 7.5g per agar plate, and the diabetic test contained 9.729g of sucrose. The thigmotaxis tests were measured in distance and time, and the β-amyloid paralysis was through count. The results seemed to show that higher concentrations led to faster thigmotaxis and slower paralysis than the control for both test types. Four two-way ANOVA tests were conducted for assurance, with the p-values being: <0.001 for hour and 0.003 for concentration in both normal and diabetic paralysis tests, and 0.002 and 0.049 for the normal thigmotaxis and diabetic thigmotaxis, respectively. Being that all these tests were less than the α=0.05, a Bonferroni test was conducted and every test was found to be significant. As the concentration of resveratrol increased, the paralysis was reduced and the thigmotaxis diminished at a slower rate.

Location

Furman Hall 209

Start Date

3-28-2020 11:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 11:00 AM

Impact of Resveratrol on the Thigmotaxis and β-amyloid Toxicity of Caenorhabditis elegans Strain Cl4176 for Diabetes and Alzheimer's Disease Applications

Furman Hall 209

Alzheimer's disease and diabetes are two of the most impactful diseases in the world, projected to be the sixth and seventh leading cause of death in the United States, respectively. The purpose of this experiment was to test resveratrol, a polyphenol, as a moderator of Alzheimer's. It was hypothesized that an increased concentration of resveratrol would increase thigmotaxis response and slow β-amyloid toxicity progression for C. elegans strain cl4176 in normal conditions and diabetic conditions (glucose toxicity). A trans-resveratrol supplement was obtained, and agar plates were set up with nematode growth agar. L4 stage C. elegans were used for each test. Resveratrol was given in concentrations of 0g, 2.5g, 5g, and 7.5g per agar plate, and the diabetic test contained 9.729g of sucrose. The thigmotaxis tests were measured in distance and time, and the β-amyloid paralysis was through count. The results seemed to show that higher concentrations led to faster thigmotaxis and slower paralysis than the control for both test types. Four two-way ANOVA tests were conducted for assurance, with the p-values being: <0.001 for hour and 0.003 for concentration in both normal and diabetic paralysis tests, and 0.002 and 0.049 for the normal thigmotaxis and diabetic thigmotaxis, respectively. Being that all these tests were less than the α=0.05, a Bonferroni test was conducted and every test was found to be significant. As the concentration of resveratrol increased, the paralysis was reduced and the thigmotaxis diminished at a slower rate.