Title

Silencing of the Wheat Gliadins and Glutenin Using the LBCPF1 Enzyme

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Botany

Presentation Type

Mentored

Abstract

Celiac Disease (CD) affects 1% of the world's population, causing patients to suffer from malnourishment, loss of bone density and even cancer. Currently, the only solution for celiac disease is lifelong abstinence from all gluten products. However, due to the mislabeling of products, social constraints, and the various gluten-containing products, this is not always possible. In the present research, we attempted to silence the four wheat prolamin groups - alpha, gamma, and omega gliadins, as well as low molecular weight (LMW) glutenins - responsible for gluten intolerance, by introducing mutations to each prolamin group. To do this, a 44 nucleotide gRNA was bound with the CRISPR enzyme LbCpf 1. The prolamin group DNA was then added, creating a target site for the LbCpf 1 enzyme to bind to. Once bound, the LbCpf1 begins to create various point mutations along the site. Results showed the reaction to be successful in silencing the alpha prolamin group from the wheat genome, however, further testing should be done on gamma, omega and LMW prolamin groups, as these were unsuccessful.

Location

Furman Hall 106

Start Date

3-28-2020 11:00 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 28th, 11:00 AM

Silencing of the Wheat Gliadins and Glutenin Using the LBCPF1 Enzyme

Furman Hall 106

Celiac Disease (CD) affects 1% of the world's population, causing patients to suffer from malnourishment, loss of bone density and even cancer. Currently, the only solution for celiac disease is lifelong abstinence from all gluten products. However, due to the mislabeling of products, social constraints, and the various gluten-containing products, this is not always possible. In the present research, we attempted to silence the four wheat prolamin groups - alpha, gamma, and omega gliadins, as well as low molecular weight (LMW) glutenins - responsible for gluten intolerance, by introducing mutations to each prolamin group. To do this, a 44 nucleotide gRNA was bound with the CRISPR enzyme LbCpf 1. The prolamin group DNA was then added, creating a target site for the LbCpf 1 enzyme to bind to. Once bound, the LbCpf1 begins to create various point mutations along the site. Results showed the reaction to be successful in silencing the alpha prolamin group from the wheat genome, however, further testing should be done on gamma, omega and LMW prolamin groups, as these were unsuccessful.