Implementing DNA Origami Dynamic Nano-Structures Into the Extracellular Matrix to Measure Interstitial Shear Force
School Name
South Carolina Governor's School for Science & Mathematics
Grade Level
12th Grade
Presentation Topic
Engineering
Presentation Type
Mentored
Abstract
Implementation of dynamic nano structures (NanoDyn) in the extra-cellular matrix (ECM) can help further the research on tumors metastasis within the ECM. Additional research of cell metastasis will improve our understanding of how and where tumors will grow, which is the first step to improving cancer treatments. Collagen is used as the environment in which the devices are placed. Peptides are used to bind the devices to the collagen. Using confocal microscopy, the NanoDyn were imaged. Single molecule microscopy was used to measure the effects of pressure on the NanoDyn devices. From the confocal, we found that between 51 degrees Celsius and 60 degrees Celsius, these devices bind best to collagen. Using C1 and C2 devices we found that they open (>180 degrees) under 50 & 100ul of pressure. These results indicate that we are able to connect these devices to the ECM and they were able to measure different pressures. The data collected can be used to help cancer researchers understand how intestinal force aids to tumor metastasis. The NanoDyn would continue to assist cancer research and help find an eventual cure.
Recommended Citation
Joseph, Sydney, "Implementing DNA Origami Dynamic Nano-Structures Into the Extracellular Matrix to Measure Interstitial Shear Force" (2020). South Carolina Junior Academy of Science. 89.
https://scholarexchange.furman.edu/scjas/2020/all/89
Location
Johns Hall 109
Start Date
3-28-2020 9:45 AM
Presentation Format
Oral Only
Group Project
No
Implementing DNA Origami Dynamic Nano-Structures Into the Extracellular Matrix to Measure Interstitial Shear Force
Johns Hall 109
Implementation of dynamic nano structures (NanoDyn) in the extra-cellular matrix (ECM) can help further the research on tumors metastasis within the ECM. Additional research of cell metastasis will improve our understanding of how and where tumors will grow, which is the first step to improving cancer treatments. Collagen is used as the environment in which the devices are placed. Peptides are used to bind the devices to the collagen. Using confocal microscopy, the NanoDyn were imaged. Single molecule microscopy was used to measure the effects of pressure on the NanoDyn devices. From the confocal, we found that between 51 degrees Celsius and 60 degrees Celsius, these devices bind best to collagen. Using C1 and C2 devices we found that they open (>180 degrees) under 50 & 100ul of pressure. These results indicate that we are able to connect these devices to the ECM and they were able to measure different pressures. The data collected can be used to help cancer researchers understand how intestinal force aids to tumor metastasis. The NanoDyn would continue to assist cancer research and help find an eventual cure.
