The Phytoremediation of Escherichia coli in Contaminated Water by Lemna minor, Salvinia minima, and Azolla caroliniana
School Name
Spring Valley High School
Grade Level
11th Grade
Presentation Topic
Microbiology
Presentation Type
Non-Mentored
Abstract
Pathogenic strains of Escherichia coli can cause potentially fatal diseases like hemorrhagic colitis. Phytoremediation is the process in which plants remove contaminants like E. coli from the environment. Lemna minor, Salvinia minima, and Azolla caroliniana are three aquatic plant species that have been tested in previous phytoremediation research, but their abilities to expunge E. coli from water have not been directly compared. The purpose of this study was to test and compare the abilities of L. minor, S. minima, and A. caroliniana to reduce E. coli concentration in contaminated water. It was hypothesized that aquatic plants would decrease the concentration of E. coli in water due to the antimicrobial flavonoids they produce, and L. minor would kill more bacteria than S. minima and A. caroliniana because of its fast growth rate and extensive roots. Plants were placed in fertilizer solution, and E. coli was added to each sample. Initial and final concentrations (CFU/mL) of E. coli in the samples were determined after a serial dilution. L. minor, S. minima, and A. caroliniana resulted in a 97.890%, 90.292%, and 99.063% decrease in E. coli concentration, respectively. A Kruskal-Wallis test found that results were statistically significant, H(3) = 51.413, p < .001, and Dunn’s pairwise tests found significant differences between L. minor vs. control, S. minima vs. control, and A. caroliniana vs. control. The results of the present study suggest that L. minor, S. minima, and A. caroliniana are equally effective at remediating E. coli-contaminated water.
Recommended Citation
Han, Madison, "The Phytoremediation of Escherichia coli in Contaminated Water by Lemna minor, Salvinia minima, and Azolla caroliniana" (2022). South Carolina Junior Academy of Science. 71.
https://scholarexchange.furman.edu/scjas/2022/all/71
Location
HSS 116
Start Date
4-2-2022 11:30 AM
Presentation Format
Oral and Written
Group Project
No
The Phytoremediation of Escherichia coli in Contaminated Water by Lemna minor, Salvinia minima, and Azolla caroliniana
HSS 116
Pathogenic strains of Escherichia coli can cause potentially fatal diseases like hemorrhagic colitis. Phytoremediation is the process in which plants remove contaminants like E. coli from the environment. Lemna minor, Salvinia minima, and Azolla caroliniana are three aquatic plant species that have been tested in previous phytoremediation research, but their abilities to expunge E. coli from water have not been directly compared. The purpose of this study was to test and compare the abilities of L. minor, S. minima, and A. caroliniana to reduce E. coli concentration in contaminated water. It was hypothesized that aquatic plants would decrease the concentration of E. coli in water due to the antimicrobial flavonoids they produce, and L. minor would kill more bacteria than S. minima and A. caroliniana because of its fast growth rate and extensive roots. Plants were placed in fertilizer solution, and E. coli was added to each sample. Initial and final concentrations (CFU/mL) of E. coli in the samples were determined after a serial dilution. L. minor, S. minima, and A. caroliniana resulted in a 97.890%, 90.292%, and 99.063% decrease in E. coli concentration, respectively. A Kruskal-Wallis test found that results were statistically significant, H(3) = 51.413, p < .001, and Dunn’s pairwise tests found significant differences between L. minor vs. control, S. minima vs. control, and A. caroliniana vs. control. The results of the present study suggest that L. minor, S. minima, and A. caroliniana are equally effective at remediating E. coli-contaminated water.
