The Effect of Pleurotus ostreatus in Bioremediation of Escherichia coli Contaminated Water Grown on Various Substrates
School Name
Spring Valley High School
Grade Level
10th Grade
Presentation Topic
Environmental Science
Presentation Type
Non-Mentored
Abstract
Bacterial contaminant in water is a major issue that causes many deaths every year (Pini & Geddes, 2020).The type of substrate the fungi grows on can greatly affect which nutrients the fungi receives which in turn affect the enzymes that it makes (El-Ramady et al., 2022). Many different things in a substrate affect the remediation rate of the fungi, such as the carbon to nitrogen ratio (El-Ramady et al., 2022). The purpose of this study was to investigate which substrate coffee, sawdust, or wheat straws, would have the highest remediation capability. It was hypothesized that if Pleurotus ostreatus grown on wheat straws, would inhibit the growth of E. coli the greatest. Compared to coffee grounds and sawdust because wheat straws have the highest percentage composition of carbohydrates meaning that they would provide the most nutrients for the fungi to grow (Gashaw et al., 2020). The fungi was grown with the substrate for three weeks, before the E. coli broth was added. After 15 days, the bacterial growth was quantitated using a colony counter. Coffee and sawdust substrate was found to have the least number of colonies than wheat straw substrate; the one-way ANOVA test shows that the data was significant F(3,116) = 45.28, p=<.0001. These findings did not support the hypothesis but rather support that sawdust and coffee provide P. ostreatus improved nutrition to inhibit E. coli growth. Therefore sawdust and coffee would be better substrates for P. ostreatus to remediation E. coli than wheat straw.
Recommended Citation
Patel, Krish, "The Effect of Pleurotus ostreatus in Bioremediation of Escherichia coli Contaminated Water Grown on Various Substrates" (2023). South Carolina Junior Academy of Science. 85.
https://scholarexchange.furman.edu/scjas/2023/all/85
Location
BS 349
Start Date
3-25-2023 9:15 AM
Presentation Format
Oral and Written
Group Project
No
The Effect of Pleurotus ostreatus in Bioremediation of Escherichia coli Contaminated Water Grown on Various Substrates
BS 349
Bacterial contaminant in water is a major issue that causes many deaths every year (Pini & Geddes, 2020).The type of substrate the fungi grows on can greatly affect which nutrients the fungi receives which in turn affect the enzymes that it makes (El-Ramady et al., 2022). Many different things in a substrate affect the remediation rate of the fungi, such as the carbon to nitrogen ratio (El-Ramady et al., 2022). The purpose of this study was to investigate which substrate coffee, sawdust, or wheat straws, would have the highest remediation capability. It was hypothesized that if Pleurotus ostreatus grown on wheat straws, would inhibit the growth of E. coli the greatest. Compared to coffee grounds and sawdust because wheat straws have the highest percentage composition of carbohydrates meaning that they would provide the most nutrients for the fungi to grow (Gashaw et al., 2020). The fungi was grown with the substrate for three weeks, before the E. coli broth was added. After 15 days, the bacterial growth was quantitated using a colony counter. Coffee and sawdust substrate was found to have the least number of colonies than wheat straw substrate; the one-way ANOVA test shows that the data was significant F(3,116) = 45.28, p=<.0001. These findings did not support the hypothesis but rather support that sawdust and coffee provide P. ostreatus improved nutrition to inhibit E. coli growth. Therefore sawdust and coffee would be better substrates for P. ostreatus to remediation E. coli than wheat straw.
