The Combined and Individual Effects of NaHCO₃ and A. indica L. oil on the Inhibition of F. oxysporum
School Name
Spring Valley High School
Grade Level
11th Grade
Presentation Topic
Microbiology
Presentation Type
Non-Mentored
Abstract
F. oxysporum is the causal agent of Fusarium wilt. This disease is known to penetrate the roots of plants and wilting the vascular system. The most common method for control is through the use of synthetic fungicides, which are known to cause adverse effects on the environment and human health. Thus, it is necessary that more methods of biocontrol be explored. Current literature shows that various salts and essential oils are effective for the biocontrol of F. oxysporum. However, the two types of substances have not yet been tested together, causing a gap in research. Due to the antifungal properties of both substances, it can be hypothesized that a combined treatment of the two would result in a synergistic effect on the F. oxysporum. 50 samples were poured into petri dishes with the treatment mixed into the agar and inoculated with agar discs containing F. oxysporum. Percent inhibition was calculated after incubation at 25±1℃ using the following equation: I = (dc-dt)/dc x 100 The results showed that the combination of sodium bicarbonate and neem oil had 73.3% as the highest inhibition rate. The positive control (acetone) had the second highest inhibition rate of 51.1%. A one-way ANOVA test (F(4,45)=95.977, p=1.1102e-16) showed that there was a statistically significant difference between at least two groups. The post-hoc Tukey test revealed significant differences between many groups, making the results unclear due to errors in the experiment.
Recommended Citation
Murugesan, Neya, "The Combined and Individual Effects of NaHCO₃ and A. indica L. oil on the Inhibition of F. oxysporum" (2023). South Carolina Junior Academy of Science. 99.
https://scholarexchange.furman.edu/scjas/2023/all/99
Location
ECL 103
Start Date
3-25-2023 10:45 AM
Presentation Format
Oral and Written
Group Project
No
The Combined and Individual Effects of NaHCO₃ and A. indica L. oil on the Inhibition of F. oxysporum
ECL 103
F. oxysporum is the causal agent of Fusarium wilt. This disease is known to penetrate the roots of plants and wilting the vascular system. The most common method for control is through the use of synthetic fungicides, which are known to cause adverse effects on the environment and human health. Thus, it is necessary that more methods of biocontrol be explored. Current literature shows that various salts and essential oils are effective for the biocontrol of F. oxysporum. However, the two types of substances have not yet been tested together, causing a gap in research. Due to the antifungal properties of both substances, it can be hypothesized that a combined treatment of the two would result in a synergistic effect on the F. oxysporum. 50 samples were poured into petri dishes with the treatment mixed into the agar and inoculated with agar discs containing F. oxysporum. Percent inhibition was calculated after incubation at 25±1℃ using the following equation: I = (dc-dt)/dc x 100 The results showed that the combination of sodium bicarbonate and neem oil had 73.3% as the highest inhibition rate. The positive control (acetone) had the second highest inhibition rate of 51.1%. A one-way ANOVA test (F(4,45)=95.977, p=1.1102e-16) showed that there was a statistically significant difference between at least two groups. The post-hoc Tukey test revealed significant differences between many groups, making the results unclear due to errors in the experiment.
