Expression of PRG in Chicken Embryos
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
PRG, which stands for plasticity-related gene, is known to modulate an axon’s response to LPA receptors. LPA (lysophosphatidic acid) causes growth cone collapse in growing axons, meaning it produces a negative signal indicating the axon to not grow in a specific direction. PRG’s response to LPA can be that it enhances the effect, reverses, etc. To find the effect of LPA on PRG, we must first find out which PRGs are present in chick embryos, which we are using as models for the human embryo. Through the process of RNA extraction and RT-PCR, we were able to determine what PRG to focus on. To extract RNA from a chick's retina, we extracted a piece of tissue from the retina in the chick embryo. Then, we flushed it with various chemicals such as trizol, chloroform, 2- propanol, and 75% ethanol to get rid of all the proteins and cell components (besides the RNA) in the tissue. We then diluted it in 50ul of water, In the RT-PCR, reverse transcription-polymerase chain reaction, we take the RNA, make it into DNA, and amplify a portion of DNA based on the primers we use. We used it to amplify the expression of PRG - 1, 2, 3, and 5. We found that PRG 3 showed up very well on the agarose gel electrophoresis. However, only faint lines appeared for PRG 1 and 2, meaning we needed to find new primers. PRG -5 was hard to locate on the gel despite using multiple types of primers. Using this information we can further determine what PRG to focus on when interacting with LPA.
Recommended Citation
Dutta, Gomahi, "Expression of PRG in Chicken Embryos" (2024). South Carolina Junior Academy of Science. 443.
https://scholarexchange.furman.edu/scjas/2024/all/443
Location
RITA 271
Start Date
3-23-2024 9:30 AM
Presentation Format
Oral Only
Group Project
No
Expression of PRG in Chicken Embryos
RITA 271
PRG, which stands for plasticity-related gene, is known to modulate an axon’s response to LPA receptors. LPA (lysophosphatidic acid) causes growth cone collapse in growing axons, meaning it produces a negative signal indicating the axon to not grow in a specific direction. PRG’s response to LPA can be that it enhances the effect, reverses, etc. To find the effect of LPA on PRG, we must first find out which PRGs are present in chick embryos, which we are using as models for the human embryo. Through the process of RNA extraction and RT-PCR, we were able to determine what PRG to focus on. To extract RNA from a chick's retina, we extracted a piece of tissue from the retina in the chick embryo. Then, we flushed it with various chemicals such as trizol, chloroform, 2- propanol, and 75% ethanol to get rid of all the proteins and cell components (besides the RNA) in the tissue. We then diluted it in 50ul of water, In the RT-PCR, reverse transcription-polymerase chain reaction, we take the RNA, make it into DNA, and amplify a portion of DNA based on the primers we use. We used it to amplify the expression of PRG - 1, 2, 3, and 5. We found that PRG 3 showed up very well on the agarose gel electrophoresis. However, only faint lines appeared for PRG 1 and 2, meaning we needed to find new primers. PRG -5 was hard to locate on the gel despite using multiple types of primers. Using this information we can further determine what PRG to focus on when interacting with LPA.
