The Bactericidal Effects of Coptis teeta Herbal Mouthwash on Streptococcus salivarius

Author(s)

Arpith GopuFollow

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

Despite the known connection of oral health to overall health, many people continue to disregard its overall significance. This study focused on chlorhexidine (CHX) mouthwashes' effect on the oral microbiome's beneficial bacteria. The purpose of this study was to determine if CHX harms beneficial bacteria and if C. teeta could be used as an alternative. The hypothesis was that a 10% C. teeta solution by weight would better encourage S. salivarius growth compared to 0.2% CHX. Previous studies showed CHX overuse leads to salivary pH fluctuation, and natural remedies could be used as alternatives. Bacteria were placed in Petri dishes containing a solution of either 10% C. teeta by weight or 0.2% CHX solution. Results showed that within 0.2% CHX killed virtually all bacteria. The C. teeta solution allowed more bacteria to grow, averaging 2.43 CFU/mL higher than CHX. Within F(2,93)=6.17, P=0.003 results were found not significant enough to suggest a difference in 10% C. teeta and 0.2% CHX antibacterial properties against S. salivarius. Showing that both C. teeta and CHX would kill beneficial bacteria, but that 10% C. teeta has very similar antibacterial properties compared to CHX.

Location

RITA 387

Start Date

3-23-2024 9:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 23rd, 9:15 AM

The Bactericidal Effects of Coptis teeta Herbal Mouthwash on Streptococcus salivarius

RITA 387

Despite the known connection of oral health to overall health, many people continue to disregard its overall significance. This study focused on chlorhexidine (CHX) mouthwashes' effect on the oral microbiome's beneficial bacteria. The purpose of this study was to determine if CHX harms beneficial bacteria and if C. teeta could be used as an alternative. The hypothesis was that a 10% C. teeta solution by weight would better encourage S. salivarius growth compared to 0.2% CHX. Previous studies showed CHX overuse leads to salivary pH fluctuation, and natural remedies could be used as alternatives. Bacteria were placed in Petri dishes containing a solution of either 10% C. teeta by weight or 0.2% CHX solution. Results showed that within 0.2% CHX killed virtually all bacteria. The C. teeta solution allowed more bacteria to grow, averaging 2.43 CFU/mL higher than CHX. Within F(2,93)=6.17, P=0.003 results were found not significant enough to suggest a difference in 10% C. teeta and 0.2% CHX antibacterial properties against S. salivarius. Showing that both C. teeta and CHX would kill beneficial bacteria, but that 10% C. teeta has very similar antibacterial properties compared to CHX.