The Effects of Ibuprofen and Methylene Blue Chloride on Mortality of Caenorhabditis elegans with the Cl2006 Strain

Arnav JaniFollow

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

Although Alzheimer’s Disease is one the most common forms of dementia and affects millions of humans around the world, there is still no discovered cure or treatment for this disease. The purpose of this experiment was to test theorized Tau and Amyloid Beta Inhibitors in order to determine if these inhibitors can minimize the effect both kinds of protein have on the cause of Alzheimer’s Disease. It was hypothesized that a group treated with both Ibuprofen and Methylene Blue Chloride (MTC) would be most successful in reducing the effects both Tau and Amyloid Beta Protein have on neurons in the brain. In order to conduct this experiment with accuracy, a specific strain of C. elegans (2006) was used to mimic Alzheimer’s Disease. A total of ten petri dishes were used and cultured with this strain of C. elegans. After a four day period, three areas from each petri dish were observed to calculate the mortality rate. After the data was collected (in percentages), an ANOVA test was performed with the alpha value being 0.05. After the statistical analysis test was performed, it was found that the p value was <0.001, indicating that the results were statistically significant. The Tukey test was also performed and the results indicated that there was significant difference in the control Ibuprofen group, the MTC group, and the group of both MTC and Ibuprofen The hypothesis was supported by the statistical analysis test run as the group with both Ibuprofen and MTC reduced the mortality the most compared to the other groups tested within this experiment. These results can be used as a basis in the search for a cure of treatment for Alziemer’s Disease.

Location

RITA 387

Start Date

3-23-2024 11:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 23rd, 11:15 AM

The Effects of Ibuprofen and Methylene Blue Chloride on Mortality of Caenorhabditis elegans with the Cl2006 Strain

RITA 387

Although Alzheimer’s Disease is one the most common forms of dementia and affects millions of humans around the world, there is still no discovered cure or treatment for this disease. The purpose of this experiment was to test theorized Tau and Amyloid Beta Inhibitors in order to determine if these inhibitors can minimize the effect both kinds of protein have on the cause of Alzheimer’s Disease. It was hypothesized that a group treated with both Ibuprofen and Methylene Blue Chloride (MTC) would be most successful in reducing the effects both Tau and Amyloid Beta Protein have on neurons in the brain. In order to conduct this experiment with accuracy, a specific strain of C. elegans (2006) was used to mimic Alzheimer’s Disease. A total of ten petri dishes were used and cultured with this strain of C. elegans. After a four day period, three areas from each petri dish were observed to calculate the mortality rate. After the data was collected (in percentages), an ANOVA test was performed with the alpha value being 0.05. After the statistical analysis test was performed, it was found that the p value was <0.001, indicating that the results were statistically significant. The Tukey test was also performed and the results indicated that there was significant difference in the control Ibuprofen group, the MTC group, and the group of both MTC and Ibuprofen The hypothesis was supported by the statistical analysis test run as the group with both Ibuprofen and MTC reduced the mortality the most compared to the other groups tested within this experiment. These results can be used as a basis in the search for a cure of treatment for Alziemer’s Disease.