The Effect of Omega-3 Fatty Acids on Locomotion in Systemic Sclerosis Model by Strain VC1927 Caenorhabditis elegans

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Abstract

The purpose of this research was to compare the results of different omega-3 fatty acids in trying to improve impaired locomotion of strain VC1927 Caenorhabditis elegans as a model for systemic sclerosis. It was hypothesized that the DHA omega-3 fatty acids would improve the locomotion of mutated C. elegans the most when compared to the EPA and ALA omega-3 fatty acids experimental groups. Procedures include the mutated C. elegans were left in the Petri dishes for one week to ensure that the nematodes were able to move through the entire agar. The VC1927 C. elegans were transferred to the different quadrant-divided Petri dishes using a scoring method. The four compartments were for the different omega-3 fatty acid and K12 E. coli solutions groups that were fed to the nematodes. The groups include alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and, what served as the control group, a plain K12 E. coli solution. The K12 E. coli solutions were made using 20 mL of K12 E. coli and 2 μg of the omega-3 fatty acids. The data acquirement began after the different 100 μL K12 E. coli solutions were fed to the corresponding mutated C. elegans using micropipettes the movement of mutated C. elegans was video recorded for 30 seconds using an iPhone held up to the lens of the stereoscope. This data was then put into the Tracker Video Analysis and Modeling Tool software. Statistical results from the one-way ANOVA test showed that F(3, 116) = 5.3482, p < 0.0017. A post-hoc Tukey test showed no significant difference between the means of the control group and the treatment groups. The results of the study indicate that omega-3 fatty acids cannot be used to treat the motility of systemic sclerosis.

Location

RITA 283

Start Date

3-23-2024 9:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 23rd, 9:30 AM

The Effect of Omega-3 Fatty Acids on Locomotion in Systemic Sclerosis Model by Strain VC1927 Caenorhabditis elegans

RITA 283

The purpose of this research was to compare the results of different omega-3 fatty acids in trying to improve impaired locomotion of strain VC1927 Caenorhabditis elegans as a model for systemic sclerosis. It was hypothesized that the DHA omega-3 fatty acids would improve the locomotion of mutated C. elegans the most when compared to the EPA and ALA omega-3 fatty acids experimental groups. Procedures include the mutated C. elegans were left in the Petri dishes for one week to ensure that the nematodes were able to move through the entire agar. The VC1927 C. elegans were transferred to the different quadrant-divided Petri dishes using a scoring method. The four compartments were for the different omega-3 fatty acid and K12 E. coli solutions groups that were fed to the nematodes. The groups include alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and, what served as the control group, a plain K12 E. coli solution. The K12 E. coli solutions were made using 20 mL of K12 E. coli and 2 μg of the omega-3 fatty acids. The data acquirement began after the different 100 μL K12 E. coli solutions were fed to the corresponding mutated C. elegans using micropipettes the movement of mutated C. elegans was video recorded for 30 seconds using an iPhone held up to the lens of the stereoscope. This data was then put into the Tracker Video Analysis and Modeling Tool software. Statistical results from the one-way ANOVA test showed that F(3, 116) = 5.3482, p < 0.0017. A post-hoc Tukey test showed no significant difference between the means of the control group and the treatment groups. The results of the study indicate that omega-3 fatty acids cannot be used to treat the motility of systemic sclerosis.