The Effect of Indole-3-Carbinol on Saccharomyces cerevisiae Growth, as a Model for Cancer Cells
School Name
Spring Valley High School
Grade Level
10th Grade
Presentation Topic
Physiology and Health
Presentation Type
Non-Mentored
Abstract
An abundance of research is conducted on cancer cells and treatments that could be used to help people who face this disease. Different concentrations of indole-3 carbinol and its impact on cancer have not been elucidated. The purpose of this study was to determine whether the concentrations of indole-3-carbinol would impact the growth of Saccharomyces cerevisiae. This data could then be applied to cancer preventatives as S. cerevisiae and cancer cells have similar growth properties. It was hypothesized that the higher the concentrations of indole-3-carbinol would exhibit more antifungal properties based on previous research showing that indole-3-carbinol tends to shut down harmful pathways, such as the Kennedy pathway. This hypothesis was tested using the following concentrations of indole-3-carbinol, 50%, 80%, and 100%. Zone of inhibition disks were placed into the solutions and placed onto the S. cerevisiae that was streaked on petri dishes. The amount of antifungal activity (in mm) was recorded. The results from the ANOVA test with a ⍺-value of 0.05 show that there were significant differences between the experimental groups and the control (p-value < 0.001). A post-Hoc-Tukey test was performed and it was found that there were significant differences between each of the experimental groups. It was concluded that there was sufficient evidence to suggest that higher concentrations of I3C led to more antifungal activity against S. cerevisiae compared to the lower concentrations.
Recommended Citation
Anchoori, Sadhana, "The Effect of Indole-3-Carbinol on Saccharomyces cerevisiae Growth, as a Model for Cancer Cells" (2024). South Carolina Junior Academy of Science. 518.
https://scholarexchange.furman.edu/scjas/2024/all/518
Location
RITA 373
Start Date
3-23-2024 9:30 AM
Presentation Format
Oral and Written
Group Project
No
The Effect of Indole-3-Carbinol on Saccharomyces cerevisiae Growth, as a Model for Cancer Cells
RITA 373
An abundance of research is conducted on cancer cells and treatments that could be used to help people who face this disease. Different concentrations of indole-3 carbinol and its impact on cancer have not been elucidated. The purpose of this study was to determine whether the concentrations of indole-3-carbinol would impact the growth of Saccharomyces cerevisiae. This data could then be applied to cancer preventatives as S. cerevisiae and cancer cells have similar growth properties. It was hypothesized that the higher the concentrations of indole-3-carbinol would exhibit more antifungal properties based on previous research showing that indole-3-carbinol tends to shut down harmful pathways, such as the Kennedy pathway. This hypothesis was tested using the following concentrations of indole-3-carbinol, 50%, 80%, and 100%. Zone of inhibition disks were placed into the solutions and placed onto the S. cerevisiae that was streaked on petri dishes. The amount of antifungal activity (in mm) was recorded. The results from the ANOVA test with a ⍺-value of 0.05 show that there were significant differences between the experimental groups and the control (p-value < 0.001). A post-Hoc-Tukey test was performed and it was found that there were significant differences between each of the experimental groups. It was concluded that there was sufficient evidence to suggest that higher concentrations of I3C led to more antifungal activity against S. cerevisiae compared to the lower concentrations.
