The Effect of Avocado Oil on the Antibacterial Effectiveness of Arthrospira platensis on Escherichia coli

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

A quarter of the meat in the US spoils before it is cooked, due to its short shelf life. Because many synthetic ways to preserve meat are shown to be hazardous to human health, there is interest in natural alternatives that avoid harmful additives. Arthrospira platensis is a cyanobacterium that possesses various inhibitory effects, such as being antibacterial, antifungal, and antioxidant. A. platensis can be used to increase the shelf life of meats through the inhibition of various bacteria. The purpose of this study was to determine if A. platensis inhibited the growth of the K-12 strain of E. coli more efficiently when used alone or combined with a lipid polyunsaturated fatty acids (PUFAs), like avocado oil. It was hypothesized that the addition of avocado oil would enhance the antimicrobial properties of Arthrospira platensis, as many of the antibacterial compounds in this species are polyunsaturated fatty acids, which are found in avocado oil and disrupt the cell walls of both Gram-positive and Gram-negative bacteria, thereby killing them. To test this, different amounts of A. platensis and avocado oil were combined on an Escherichia coli bacterial lawn, and the zone of inhibition after 24 hours of incubation was measured. No treatment showed any zone of inhibition, not supporting the hypothesis. Additional testing is needed to adequately defend or refute the hypothesis as no viable data was gathered.

Location

Furman Hall 111

Start Date

3-28-2026 10:14 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 10:14 AM

The Effect of Avocado Oil on the Antibacterial Effectiveness of Arthrospira platensis on Escherichia coli

Furman Hall 111

A quarter of the meat in the US spoils before it is cooked, due to its short shelf life. Because many synthetic ways to preserve meat are shown to be hazardous to human health, there is interest in natural alternatives that avoid harmful additives. Arthrospira platensis is a cyanobacterium that possesses various inhibitory effects, such as being antibacterial, antifungal, and antioxidant. A. platensis can be used to increase the shelf life of meats through the inhibition of various bacteria. The purpose of this study was to determine if A. platensis inhibited the growth of the K-12 strain of E. coli more efficiently when used alone or combined with a lipid polyunsaturated fatty acids (PUFAs), like avocado oil. It was hypothesized that the addition of avocado oil would enhance the antimicrobial properties of Arthrospira platensis, as many of the antibacterial compounds in this species are polyunsaturated fatty acids, which are found in avocado oil and disrupt the cell walls of both Gram-positive and Gram-negative bacteria, thereby killing them. To test this, different amounts of A. platensis and avocado oil were combined on an Escherichia coli bacterial lawn, and the zone of inhibition after 24 hours of incubation was measured. No treatment showed any zone of inhibition, not supporting the hypothesis. Additional testing is needed to adequately defend or refute the hypothesis as no viable data was gathered.